Laboratory Information

NameLP View on WormBase
Allele designationcp
HeadBob Goldstein
InstitutionUniversity of North Carolina, Chapel Hill, NC
Address 616 Fordham Hall
UNC-CH Biology
Chapel Hill 27599-3280
United States
Website http://www.bio.unc.edu/faculty/goldstein/
Gene classes gadr  gfl 

Strains contributed by this laboratory

Strain Genotype Species Description
LP162 nmy-2(cp13[nmy-2::GFP + LoxP]) I. C. elegans cp13[nmy-2::gfp + LoxP] I. GFP inserted into the endogenous nmy-2 gene by Cas9-triggered homologous recombination. Green fluorescence in many tissues, especially the germline. Fluorescence is similar to, but brighter than, the nmy-2::GFP transgene zuIs45. Reference: Dickinson DJ, et al. 2013 Nature Methods Advance Online Publication. DOI: 10.1038/nmeth.2641.
LP172 hmr-1(cp21[hmr-1::GFP + LoxP]) I. C. elegans cp21[hmr-1::GFP + LoxP] I. GFP inserted at the C terminus of endogenous hmr-1 gene by Cas9-triggered homologous recombination. Floxed unc-119 selection cassette was subsequently removed by Cre/Lox recombination leaving a LoxP scar after the 3'UTR. GFP expression in early embryos, larvae, and adults. Reference: Marston DJ, et al. Curr Biol. 2016 26:2079-2089.
LP176 unc-119(ed3) III; che-12(cp25[che-12::GFP + LoxP + unc-119(+) + LoxP]) V. C. elegans N-terminally tagged GFP::CHE-12. Cilia are slightly shorter than WT. GFP fluorescence appears in multiple sensory cilia in the head and phasmid neuron cilia in the tail. Reference: Das A, et al. Mol Biol Cell. 2015 Nov 15;26(23):4248-64.
LP177 unc-119(ed3) III; che-12(cp26[GFP + LoxP + unc-119(+) + LoxP]) V. C. elegans Entire che-12 coding sequence deleted and replaced with GFP. This produced a phenotype that is more penetrant than that reported for the original che-12 strain, which introduced a nonsense mutation midway through the coding region. Short amphid and phasmid cilia. Defective chemotaxis to NaCl. Dye-filing defective. Reference: Das A, et al. Mol Biol Cell. 2015 Nov 15;26(23):4248-64.
LP184 mom-5(cp31[mom-5::YPET + LoxP]) I. C. elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP191 unc-119(ed3) III; hmp-1(cp20[hmp-1::GFP + LoxP unc-119(+) LoxP]) V. C. elegans cp20[hmp-1::gfp + LoxP] V. GFP inserted at the C terminus of endogenous hmp-1 gene by Cas9-triggered homologous recombination. Green fluorescence in early embryos, larvae, and adults. Reference: Marston DJ, et al. Curr Biol. 2016 26:2079-2089.
LP193 cpIs56 II; unc-119(ed3) III. C. elegans cpIs56 [mex-5p::TagRFP-T::PLC(delta)-PH::tbb-2 3'UTR + unc-119 (+)] II. Reporter labels plasma membrane in early embryo. Transgene construct consisted of a germline promoter sequence (mex-5) driving the expression of a fluorescent protein fused to the N-terminus of the the pleckstrin homology domain from phospholipase C-(delta)1 (PH domain) and a 2x Flag epitope tag. Reference: Heppert JK, et al. Mol Biol Cell. 2016 Nov 7;27(22):3385-3394.
LP198 unc-119(ed3) III; che-12(cp34[gfp::che-12 + LoxP + unc-119(+) + LoxP]) V. C. elegans C-terminally tagged CHE-12::GFP. Cilia are slightly shorter than WT. GFP fluorescence appears in multiple sensory cilia in the head and phasmid neuron cilia in the tail. Reference: Das A, et al. Mol Biol Cell. 2015 Nov 15;26(23):4248-64.
LP212 pkc-3(cp41[mNeonGreen:3xFlag::pkc-3 + LoxP]) II. C. elegans mNG::aPKC endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947).
LP216 par-6(cp45[par-6::mNeonGreen::3xFlag + LoxP unc-119(+) LoxP]) I; unc-119(ed3) III. C. elegans PAR-6::mNG endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947).
LP228 dsh-2(cp51[mNeonGreen::dsh-2]) II. C. elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP242 par-3(cp54[mNeonGreen::3xFlag::par-3]) III. C. elegans mNG::PAR-3 endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947).
LP244 par-6(cp60[par-6::mKate2::3xMyc + LoxP unc-119(+) LoxP]) I; unc-119(ed3) III. C. elegans PAR-6::mKate2 endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947).
LP252 mrck-1(cp65[mrck-1::YPet + LoxP]) V. C. elegans cp65[mrck-1::YPet + LoxP]. YPet inserted at the C terminus of endogenous mrck-1 gene by Cas9-triggered homologous recombination. Floxed unc-119 selection cassette was subsequently removed by Cre/Lox recombination leaving a LoxP scar after the 3'UTR. Yellow fluorescence in early embryos, larvae, and adults. Reference: Marston DJ, et al. Curr Biol. 2016 26:2079-2089.
LP256 nmy-2(cp69[nmy-2::mkate2 + LoxP]) I. C. elegans NMY-2::mKate2 endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947).
LP262 cpEx25. C. elegans cpEx25 [mrck-1(delta CRIB)::YPet + myo-2p::mCherry]. Pick mCherry+ animals to maintain array. YPet-tagged MRCK-1 truncated upstream of the CRIB domain to inhibit binding to CDC-42. Generated in N2 background. Reference: Marston DJ, et al. Curr Biol. 2016 26:2079-2089.
LP306 cpIs53 II; unc-119(ed3) III. C. elegans cpIs53 [mex-5p::GFP-C1::PLC(delta)-PH::tbb-2 3'UTR + unc-119 (+)] II. Reporter labels plasma membrane in early embryo. Transgene construct consisted of a germline promoter sequence (mex-5) driving the expression of a fluorescent protein fused to the N-terminus of the pleckstrin homology domain from phospholipase C-(delta)1 (PH domain) and a 2x Flag epitope tag. Reference: Heppert JK, et al. Mol Biol Cell. 2016 Nov 7;27(22):3385-3394.
LP307 cpIs54 II; unc-119(ed3) III. C. elegans cpIs54 [mex-5p::mKate::PLC(delta)-PH(A735T)::tbb-2 3'UTR + unc-119 (+)] II. Reporter labels plasma membrane in early embryo. Transgene construct consisted of a germline promoter sequence (mex-5) driving the expression of a fluorescent protein fused to the N-terminus of the pleckstrin homology domain from phospholipase C-(delta)1 (PH domain) and a 2x Flag epitope tag. Reference: Heppert JK, et al. Mol Biol Cell. 2016 Nov 7;27(22):3385-3394.
LP308 cpIs55 II; unc-119(ed3) III. C. elegans cpIs55 [mex-5p::mCherry-C1::PLC(delta)-PH::tbb-2 3'UTR + unc-119 (+)] II. Reporter labels plasma membrane in early embryo. Transgene construct consisted of a germline promoter sequence (mex-5) driving the expression of a fluorescent protein fused to the N-terminus of the pleckstrin homology domain from phospholipase C-(delta)1 (PH domain) and a 2x Flag epitope tag. Reference: Heppert JK, et al. Mol Biol Cell. 2016 Nov 7;27(22):3385-3394.
LP316 hmp-2(cp78[GFP::hmp-2a + LoxP]) III. C. elegans cp78[gfp::hmp-2 + LoxP] III. GFP inserted at the N terminus of endogenous hmp-2 gene by Cas9-triggered homologous recombination. Floxed unc-119 selection cassette was subsequently removed by Cre/Lox recombination leaving a LoxP scar in the second synthetic intron of GFP. Green fluorescence in early embryos, larvae, and adults. Reference: Marston DJ, et al. Curr Biol. 2016 26:2079-2089.
LP322 nmy-2(ne3409) hmr-1(cp21[hmr-1::GFP + LoxP]) I. C. elegans Maintain at 15C. Semi-permissive at 20C. 100% lethal at 25C. cp21[hmr-1::gfp + LoxP] I males were crossed to WM179 nmy-2(ne3409) hermaphrodites. Fluorescence in early embryos, larvae, and adults. Severe cytokinesis defects in early embryos at 25°C. Reference: Marston DJ, et al. Curr Biol. 2016 26:2079-2089.
LP362 gex-3(cp114[mNG-C1^3xFlag::gex-3]) IV. C. elegans mNG:GEX-3 endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947).
LP373 mex-5(cp125[mNG-C1^3xFlag::mex-5]) IV. C. elegans mNG::MEX-5 endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947).
LP393 oma-2(cp145[mNG-C1^3xFlag::oma-2]) V. C. elegans mNG::OMA-2 endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947).
LP399 rap-1(cp151[mNG-C1^3xFlag::rap-1]) IV. C. elegans mNG::RAP-1 endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947).
LP402 cpIs64 II; unc-119(ed3) III. C. elegans cpIs64 [mex-5p::mYPet::PLC(delta)-PH::tbb-2 3'UTR + unc-119 (+)] II. Reporter labels plasma membrane in early embryo. Transgene construct consisted of a germline promoter sequence (mex-5) driving the expression of a fluorescent protein fused to the N-terminus of the pleckstrin homology domain from phospholipase C-(delta)1 (PH domain) and a 2x Flag epitope tag. Reference: Heppert JK, et al. Mol Biol Cell. 2016 Nov 7;27(22):3385-3394.
LP435 apr-1(cp166[mNG-C1^3xFlag::apr-1]) I. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP439 nud-2(cp170[nud-2::mNG-C1^3xFlag]) I. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP443 klp-17(cp174[klp-17::mNG-C1^3xFlag]) II. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP447 klp-7(cp178[klp-7::mNG-C1^3xFlag]) III. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP451 bicd-1(cp180[mNG-C1^3xFlag::bicd-1]) IV. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP462 mrck-1(cp189[mrck-1::GFP::3xFlag]) V. C. elegans cp189[mrck-1::GFP::3xFlag]. GFP inserted at the C terminus of endogenous mrck-1 gene by Cas9-triggered homologous recombination. Floxed selection cassette was subsequently removed by Cre/Lox recombination leaving a LoxP scar after the 3'UTR. GFP expression in early embryos, larvae, and adults. Reference: Marston DJ, et al. Curr Biol. 2016 26:2079-2089.
LP521 klp-12(cp234[klp-12::mNG-C1^3xFlag]) IV. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP527 mes-1(cp240[mes-1::mNG-C1^3xFlag] X. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP530 cam-1(cp243[cam-1::mNG-C1^3xFlag]) II. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP538 gsk-3(cp251[gsk-3::mNG-C1^3xFlag]) I. C. elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP559 mom-2(cp267[mom-2::mNG-C1^3xFlag]) V. C. elegans FP fusion. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP560 dhc-1(cp268[dhc::mNG-C1::3xFlag]) I. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP563 dnc-1(cp271[dnc::mNG-C1::3xFlag]) IV. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP585 lin-5(cp288[lin-5::mNG-C1^3xFlag]) II. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP591 lis-1(cp294[lis-1::mNG-C1^3xFlag]) III. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP598 dlg-1(cp301[dlg-1::mNG-C1^3xFlag]) X. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP600 klp-4(cp303[klp-4::mNG-C1^3xFlag]) X. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP604 klp-8(cp307[klp-8::mNG-C1^3xFlag]) X. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP608 klp-20(cp311[klp-20::mNG-C1^3xFlag]) III. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP621 par-3(cp323[HaloTag-GLO^PAR-3]) III. C. elegans Halo::PAR-3 endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947).
LP634 pkc-3(cp328[HaloTag-GLO^PKC-3]) II. C. elegans Halo::aPKC endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947).
LP637 par-2(cp329[mNG-C1^PAR-2]) III. C. elegans mNG::PAR-2 endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947).
LP653 par-6(cp345[par-6::HaloTag-GLO]) I. C. elegans PAR-6::Halo endogenously tagged using Cas9-triggered homologous recombination. Reference: Dickinson DJ, et al. Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID 28829947).
LP697 mom-5(cp367[mom-5::mNG-C1^3xFlag]) I. C. elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP705 dsh-1(cp375[dsh-1DIX::mNG-C1^3xFlag::PDZ,DEP]) II. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP713 pry-1(cp383[pry-1::mNG-C1^3xFlag]) I. C elegans FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP728 mig-5(cp385[mNG-GLO::AID::mig-5]) II. C elegans FP knock-in. [NOTE: (4/1/2021) A lab has reported finding a second GFP insertion in LP728; it has not yet been confirmed whether or not it is present in the current CGC stock.] Reference: Heppert JK, et al. 2017 Genetics. In press.
LP847 lea-1(cp423[myo-2p::GFP::myo-2 3’UTR]) V. C. elegans Null allele of lea-1. lea-1 gene replaced with myo-2p::GFP reporter. cp423 mutants can be identified by GFP expression in pharynx. Reference: Hibshman JD & Goldstein B. BMC Biol. 2021 Dec 14;19(1):263. doi: 10.1186/s12915-021-01176-0. PMID: 34903234
LP852 daf-2(e1370) III; lea-1(cp423[myo-2p::GFP::myo-2 3’UTR]) V. C. elegans Maintain at 15C. Null allele of lea-1. lea-1 gene replaced with myo-2p::GFP reporter. cp423 mutants can be identified by GFP expression in pharynx. Reference: Hibshman JD & Goldstein B. BMC Biol. 2021 Dec 14;19(1):263. doi: 10.1186/s12915-021-01176-0. PMID: 34903234
LP858 lea-1(cp431[mNG::3x FLAG::AID::lea-1]) V. C. elegans Endogenous LEA-1 tagged at the C-terminus with mNG and AID* sequence for auxin-induced degradation. Reference: Hibshman JD & Goldstein B. BMC Biol. 2021 Dec 14;19(1):263. doi: 10.1186/s12915-021-01176-0. PMID: 34903234
LP859 lea-1(cp430[lea-1::mYPet::3x FLAG]) V. C. elegans Endogenous LEA-1 tagged at the C-terminus with mYPet. Reference: Hibshman JD & Goldstein B. BMC Biol. 2021 Dec 14;19(1):263. doi: 10.1186/s12915-021-01176-0. PMID: 34903234
LP860 daf-2(e1370) III; lea-1(cp431[mNG::3x FLAG::AID::lea-1]) V. C. elegans Endogenous LEA-1 tagged at the C-terminus with mNG and AID* sequence for auxin-induced degradation. Maintain at 15C. Reference: Hibshman JD & Goldstein B. BMC Biol. 2021 Dec 14;19(1):263. doi: 10.1186/s12915-021-01176-0. PMID: 34903234
LP861 daf-2(e1370) III; lea-1(cp430[lea-1::mYPET::3x FLAG]) V. C. elegans Endogenous LEA-1 tagged at the C-terminus with mYPET. Maintain at 15C. Reference: Hibshman JD & Goldstein B. BMC Biol. 2021 Dec 14;19(1):263. doi: 10.1186/s12915-021-01176-0. PMID: 34903234
LP869 cpSi171 I. C. elegans cpSi171 [vha-8p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in multiple tissues including intestine, hypodermis, and excretory cell.
LP870 cpSi172 I. C. elegans cpSi172 [myo-2p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in the pharynx.
LP871 cpSi174 I. C. elegans cpSi174 [myo-3p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in body wall muscle.
This laboratory hasn't submitted any alleles to the CGC.