Strain Information

Name LN170   View On Wormbase
Species C. elegans
Genotyperpn-6.2(rc3[GFP + SEC::rpn-6.2b]) III.
DescriptionRoller. Reduced brood size and reduced sperm count. CRISPR/Cas9 GFP insertion at amino acid 216 of RPN-6.2a. Expression of GFP in the spermatogenic germline. The SEC (self excising cassette) contains the stop codon and transcriptional termination signal for GFP as well as a sqt-1(d) allele. Pick rollers to maintain the strain with the SEC. Excision will result in an in frame fusion of GFP at amino acid 216 of RPN-6.2a or amino acid 5 of RPN-6.2b.
Made byLynn Boyd
Laboratory LN
Reference Currently unpublished. We are working on the manuscript.
Sign in or register an account if you want to order this strain.