Strain Information
| Name | LN170 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | rpn-6.2(rc3[GFP + SEC::rpn-6.2b]) III. |
| Description | Roller. Reduced brood size and reduced sperm count. CRISPR/Cas9 GFP insertion at amino acid 216 of RPN-6.2a. Expression of GFP in the spermatogenic germline. The SEC (self excising cassette) contains the stop codon and transcriptional termination signal for GFP as well as a sqt-1(d) allele. Pick rollers to maintain the strain with the SEC. Excision will result in an in frame fusion of GFP at amino acid 216 of RPN-6.2a or amino acid 5 of RPN-6.2b. |
| Mutagen | |
| Outcrossed | x0 |
| Made by | Lynn Boyd |
| Laboratory | LN |
| Reference | Currently unpublished. We are working on the manuscript. |
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