Gene Information: rpn-6.2
| Name | rpn-6.2 View on WormBase |
|---|---|
| Species | C. elegans |
| Sequence | F59B2.5 |
| Genetic position | III:0.06 +/- 0.001 cM |
| Genomic position | III: 8999817..9001999 |
Strains carrying this gene
| Strain | Genotype | Description |
|---|---|---|
| LN167 | rpn-6.2(rc2[rpn-6.2::GFP]) III. | RPN-6.2 is a sperm-specific proteasome subunit. CRISPR/Cas9 GFP insertion. Robust RPN-6.2::GFP expression in developing and mature sperm. |
| LN170 | rpn-6.2(rc3[GFP + SEC::rpn-6.2b]) III. | Roller. Reduced brood size and reduced sperm count. CRISPR/Cas9 GFP insertion at amino acid 216 of RPN-6.2a. Expression of GFP in the spermatogenic germline. The SEC (self excising cassette) contains the stop codon and transcriptional termination signal for GFP as well as a sqt-1(d) allele. Pick rollers to maintain the strain with the SEC. Excision will result in an in frame fusion of GFP at amino acid 216 of RPN-6.2a or amino acid 5 of RPN-6.2b. |