Strain Information
| Name | KX155 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | ife-1(eu20[mKate2:Myc3x:ife-1]) III, ife-3(eu21[GFP::Flag3x::ife-3]) V |
| Description | In-frame CRISPR/Cas9 fusions into the genes encoding the eIF4E paralogs IFE-1 and IFE-3. Red and Green fluorescence, respectively. Strong fluorescence for both in the hermaphrodite and male gonads, but with distinct expression character and germ granule association. Germ cell cytoplasmic expression and lesser somatic expression are also observed. Both insertions are homozygous by PCR confirmation. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x2 |
| Made by | Hayden Huggins |
| Laboratory | KX |
| Reference | 1. Distinct roles of two eIF4E isoforms in the germline of Caenorhabditis elegans. Huggins HP, Subash JS, Stoffel H, Henderson MA, Hoffman JL, Buckner DS, Sengupta MS, Boag PR, Lee MH, Keiper BD. J Cell Sci. 2020 Mar 30;133(6):jcs237990. doi: 10.1242/jcs.237990. PMID: 32079657. 2. Two germ granule eIF4E isoforms reside in different mRNPs to hand off C elegans mRNAs from translational repression to activation. Gajjar G, Huggins HP, Kim ES, Huang W, Bonnet FX, Updike DL, Keiper BD. bioRxiv [Preprint]. 2024 May 26:2024.05.24.595216. doi: 10.1101/2024.05.24.595216. PMID: 38826235. |
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