Strain Information
| Name | KRA868 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | tol-1(syb8810[tol-1 Q712A,Y713A,G714A,N715A] *syb8406) I/+; lat-1(syb8955[lat-1 F69A] *syb8408) II. |
| Description | CRISPR/Cas9-engineered mutation of residues that mediate interaction with TOL-1 receptor in development; syb8406 is WrmScarlet and 3xFlag tags inserted into endogenous tol-1 locus. Engineered F69A mutation in endogenously-tagged lat-1 locus; syb8408 is internal eGFP and FLAG tags with poly-A linkers inserted into endogenous lat-1 locus before 651 aa. Homozygous lat-1(syb8955;syb8408) animals harboring heterozygous tol-1(syb8810;syb8406)/+. Animals exhibit reduced brood sizes and delayed development. Animals segregate into lat-1(syb8955;syb8408);tol-1(syb8810;syb8406) homozygotes, which are lethal; lat-1(syb8955;syb8408);tol-1(syb8810;syb8406)/+ heterozygotes, and lat-1(syb8955;syb8408);+/+, which have slightly reduced brood sizes. To maintain, pick EGFP(+) progeny. |
| Mutagen | No mutagen |
| Outcrossed | x1 |
| Made by | Jayson J Smith |
| Laboratory | KRA |
| Reference | Carmona-Rosas, Li, Smith et al. "Structural basis and functional roles for Toll-like receptor binding to Latrophilin adhesion-GPCR in embryo development." bioRxiv (2023) doi: https://doi.org/10.1101/2023.05.04.539414 |
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