Strain Information
| Name | JM313 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | emr-1(gk119) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); lem-2(ca19) II. |
| Description | emr-1(gk119) heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP emr-1(gk119); lem-2(ca19) homozygotes (maternal rescue). Homozygous hT2 [bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. 1/16 of offspring will be emr-1(gk119) I; lem-2(ca19) homozygotes that are maternally rescued by the single copy of emr-1 on the hT2 balancer. ca19 is a Leu to Arg mutation at position 16 of LEM-2, reconstituting a mutation in the American Hutterite Population that causes juvenile cataracts and premature cardiomyopathy. qIs48 contains [myo-2p::GFP + pes-10p::GFP + ges-1p::GFP] III. |
| Mutagen | No mutagen |
| Outcrossed | x0 |
| Made by | Ayaa AlKhaleefa |
| Laboratory | JM |
| Reference | We will be submitting a micropublication (Al Khaleefa, Snider, Duff and McGhee) describing this strain and why it is interesting. |
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