Laboratory Information

NameJM View on WormBase
Allele designationca
HeadMcGhee, Jim
InstitutionUniversity of Calgary, Alberta
Address University of Calgary
BMB/ACHRI HSC2209
3330 Hospital Drive NW
Calgary T2N 4N1
Canada
Website http://www.ucalgary.ca/~jmcghee
Gene classes erl  fkh  ges  gob  pho  tasp 

Strains contributed by this laboratory

Strain Genotype Species Description
JM1041 ges-1(ca6ca7) V. C. elegans Phenotypically normal except that it does not show ges-1 activity. Mutations (ca6ca7) are presumably misense or nonsense but have not been characterized genetically.
JM124 elt-4(ca16) X. C. elegans No obvious phenotype. Chromosomal deletion beginning from -42 bp to +1196 bps relative to elt-4 ATG (+20bp insert).
JM125 caIs108. C. elegans caIs108 [ges-1p::YFP::actin + unc-119(+)].
JM126 pho-1(ca101ca102) II. C. elegans Partial maternal effect lethal. Lack of PHO-1 acid phosphatase activity on isoelectric focusing gel.
JM130 pho-1(ca101ca102) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozgyotes are WT and segregate WT, DpyUncs, and Unc Pho (slightly slow growing, about 15% shorter than WT and produce about 60% inviable embryos; early broods are less viable than later broods).
JM144 mnDp1 (X;V)/+ V; lin-15B&lin-15A(n765) gob-1(ca17) X. C. elegans Animals with Dp/+ are WT. Animals which have lost the Dp arrest as L1 with Gob (gut-obstructed) phenotype. Animals with Dp/Dp are sterile homozygotes. gob-1(ca17) is a small deletion, removing nine genes between R03A10.4 and H13N06.4 (inclusive) and possibly the seven additional genes F39D8.2 to R03A10.3 and H13N05.6.
JM149 caIs71. C. elegans caIs71[elt-2p::GFP::HIS-2B::unc-54 3'UTR + rol-6(su1006)]. Rollers. Expresses nuclear-localized GFP in all intestinal nuclei under control of 5.2 kb elt-2 promoter. GFP is fused to Histone H2B + (fused pie-1 and truncated unc-54 3'-UTR). Transgene was integrated into N2 background by exposure to gamma rays. Reference: Dineen A, et al. Dev Biol. 2018 Mar 15;435(2):150-161. PMID: 29360433
JM311 lem-2(ca19) II. C. elegans Overall healthy but reduced brood size and pharyngeal pumping rate. Synthetic lethal with emr-1(-). ca19 is a Leu to Arg mutation at position 16 of LEM-2, reconstituting a mutation in the American Hutterite Population that causes juvenile cataracts and premature cardiomyopathy.
JM312 emr-1(gk119) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); lem-2(ok1807) II. C. elegans emr-1(gk119) heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP emr-1(gk119); lem-2(ok1807) homozygotes (partial maternal rescue; arrest ~L2 larvae). Homozygous hT2 [bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. 1/16 of offspring will be emr-1(gk119) I; lem-2(ca19) homozygotes that are partially maternally rescued by the single copy of emr-1 on the hT2 balancer. qIs48 contains [myo-2p::GFP + pes-10p::GFP + ges-1p::GFP] III. This is a control strain for JM313.
JM313 emr-1(gk119) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); lem-2(ca19) II. C. elegans emr-1(gk119) heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP emr-1(gk119); lem-2(ca19) homozygotes (maternal rescue). Homozygous hT2 [bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. 1/16 of offspring will be emr-1(gk119) I; lem-2(ca19) homozygotes that are maternally rescued by the single copy of emr-1 on the hT2 balancer. ca19 is a Leu to Arg mutation at position 16 of LEM-2, reconstituting a mutation in the American Hutterite Population that causes juvenile cataracts and premature cardiomyopathy. qIs48 contains [myo-2p::GFP + pes-10p::GFP + ges-1p::GFP] III.
JM47 ges-1(ca6) V. C. elegans Phenotypically WT. Isoelectric variant of ges-1 intestinal carboxylesterase.
JM69 dpy-5(e61) unc-13(e1091)/szT1 [lon-2(e678)] I; elt-2(ca15)/szT1 X. C. elegans Heterozygotes are WT and segregate WT, Lon males and dead eggs.
JM9 ges-1(ca1) V. C. elegans Phenotypically WT. Isoelectric variant of ges-1 intestinal carboxylesterase.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
ca6 Allele
ca7 Allele
ca16 Allele insertion
ca101 Allele substitution
ca102 Allele substitution
ca17 Allele
ca15 Allele deletion
ca1 Allele