Laboratory Information
| Name | JM View on WormBase |
|---|---|
| Allele designation | ca |
| Head | McGhee, Jim |
| Institution | University of Calgary, Alberta |
| Address | University of Calgary BMB/ACHRI HSC2209 3330 Hospital Drive NW Calgary T2N 4N1 Canada |
| Website | http://www.ucalgary.ca/~jmcghee |
| Gene classes | erl fkh ges gob pho tasp |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| JM1041 | ges-1(ca6ca7) V. | C. elegans | Phenotypically normal except that it does not show ges-1 activity. Mutations (ca6ca7) are presumably misense or nonsense but have not been characterized genetically. |
| JM124 | elt-4(ca16) X. | C. elegans | No obvious phenotype. Chromosomal deletion beginning from -42 bp to +1196 bps relative to elt-4 ATG (+20bp insert). |
| JM125 | caIs108. | C. elegans | caIs108 [ges-1p::YFP::actin + unc-119(+)]. |
| JM126 | pho-1(ca101ca102) II. | C. elegans | Partial maternal effect lethal. Lack of PHO-1 acid phosphatase activity on isoelectric focusing gel. |
| JM130 | pho-1(ca101ca102) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. | C. elegans | Heterozgyotes are WT and segregate WT, DpyUncs, and Unc Pho (slightly slow growing, about 15% shorter than WT and produce about 60% inviable embryos; early broods are less viable than later broods). |
| JM144 | mnDp1 (X;V)/+ V; lin-15B&lin-15A(n765) gob-1(ca17) X. | C. elegans | Animals with Dp/+ are WT. Animals which have lost the Dp arrest as L1 with Gob (gut-obstructed) phenotype. Animals with Dp/Dp are sterile homozygotes. gob-1(ca17) is a small deletion, removing nine genes between R03A10.4 and H13N06.4 (inclusive) and possibly the seven additional genes F39D8.2 to R03A10.3 and H13N05.6. |
| JM149 | caIs71. | C. elegans | caIs71[elt-2p::GFP::HIS-2B::unc-54 3'UTR + rol-6(su1006)]. Rollers. Expresses nuclear-localized GFP in all intestinal nuclei under control of 5.2 kb elt-2 promoter. GFP is fused to Histone H2B + (fused pie-1 and truncated unc-54 3'-UTR). Transgene was integrated into N2 background by exposure to gamma rays. Reference: Dineen A, et al. Dev Biol. 2018 Mar 15;435(2):150-161. PMID: 29360433 |
| JM311 | lem-2(ca19) II. | C. elegans | Overall healthy but reduced brood size and pharyngeal pumping rate. Synthetic lethal with emr-1(-). ca19 is a Leu to Arg mutation at position 16 of LEM-2, reconstituting a mutation in the American Hutterite Population that causes juvenile cataracts and premature cardiomyopathy. |
| JM47 | ges-1(ca6) V. | C. elegans | Phenotypically WT. Isoelectric variant of ges-1 intestinal carboxylesterase. |
| JM69 | dpy-5(e61) unc-13(e1091)/szT1 [lon-2(e678)] I; elt-2(ca15)/szT1 X. | C. elegans | Heterozygotes are WT and segregate WT, Lon males and dead eggs. |
| JM9 | ges-1(ca1) V. | C. elegans | Phenotypically WT. Isoelectric variant of ges-1 intestinal carboxylesterase. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| ca6 | Allele | ||
| ca7 | Allele | ||
| ca16 | Allele | insertion | |
| ca101 | Allele | substitution | |
| ca102 | Allele | substitution | |
| ca17 | Allele | ||
| ca15 | Allele | deletion | |
| ca1 | Allele |