Strain Information
| Name | JK6578 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | fbf-1(ok91) fbf-2(q1261[*q973])/mIn1 [mIs14 dpy-10(e128)] II. |
| Description | Pick wild-type GFP+ to maintain. 3xFlag tag inserted into endogenous fbf-2 locus with engineered (H453A H454A E457A) substitutions. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-1 fbf-2 homozygotes (Sterile). Pick WT dim GFP and check for correct segregation of progeny to maintain. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Brian Carrick |
| Laboratory | JK |
| Reference | unpublished |
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