Strain Information

Name JK6547   View On Wormbase
Species C. elegans
Genotypefbf-1(q1250) fbf-2(q738)/mIn1 [mIs14 dpy-10(e128)] II.
DescriptionPick wild-type GFP+ to maintain. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-1 fbf-2 homozygotes (sterile). Pick WT dim GFP and check for correct segregation of progeny to maintain. q1250 is an engineered Y477A point mutation in FBF-1 derived by modification of parental strain JK3101.
MutagenCrispr/Cas9
Outcrossedx2
Made byBrian Carrick
Laboratory JK
Reference unpublished
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