Strain Information
| Name | JH3479 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | meg-3(ax3056[del(545-862)::OLLAS]) meg-4(ax3052) X. |
| Description | Embryonic P granules not localized, 30% maternal effect sterility on average, RNAi insensitivity. meg-3(ax3056) is an in-frame deletion in the endogenous meg-3 locus removing amino acids (545-862); MEG-3 forms a cytoplasmic gradient but does not form granules in early embryos or recruit other P granule components such as PGL-1/3. References: Smith, J. et al. Elife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198 Schmidt H, bioRxiv 2020.10.15.340570 (2020) doi:10.1101/2020.10.15.340570. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Deepika Calidas |
| Laboratory | JH |
| Reference | Smith, J. et al. Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3. Elife 5, (2016). Schmidt, H., Putnam, A., Rasoloson, D. & Seydoux, G. Coordination of RNA and protein condensation by the P granule protein MEG-3. bioRxiv 2020.10.15.340570 (2020) doi:10.1101/2020.10.15.340570. |
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