Strain Information

Name JH3479   View On Wormbase
Species C. elegans
Genotypemeg-3(ax3056[del(1-544)::OLLAS]) meg-4(ax3052) X.
DescriptionEmbryonic P granules not localized, 30% maternal effect sterility on average, insensitivity to RNAi. meg-3(ax3056) is an-frame deletion in the endogenous meg-3 locus removing the first 544 amino acids (1-544); MEG-3 forms a cytoplasmic gradient but does not form granules in the early embryos or recruit other P granule components such as PGL-1/3. References: Smith, J. et al. Elife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198 Schmidt H, bioRxiv 2020.10.15.340570 (2020) doi:10.1101/2020.10.15.340570.
MutagenCrispr/Cas9
Outcrossedx0
Made byDeepika Calidas
Laboratory JH
Reference Smith, J. et al. Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3. Elife 5, (2016). Schmidt, H., Putnam, A., Rasoloson, D. & Seydoux, G. Coordination of RNA and protein condensation by the P granule protein MEG-3. bioRxiv 2020.10.15.340570 (2020) doi:10.1101/2020.10.15.340570.
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