Strain Information

Name JH3475   View On Wormbase
Species C. elegans
Genotypemeg-3(ax3055) meg-4(ax3052) X.
DescriptionEmbryonic P granules not segregated, 30% maternal effect sterility on average, RNAi insensitivity. meg-3(ax3055) and meg-4(ax3052) are precise deletions of the entire coding sequence made by CRISPR/Cas9, designed to be cut again to make insertions at the endogenous locus. References: Smith, J. et al. Elife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198 Ouyang JPT, et al. Dev Cell. 2019 Sep 23;50(6):716-728.e6. PMID: 31402283
MutagenCrispr/Cas9
Outcrossedx0
Made byHelen Schmidt
Laboratory JH
Reference Smith, J. et al. Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3. Elife 5, (2016). Ouyang, J. P. T. et al. P Granules Protect RNA Interference Genes from Silencing by piRNAs. Dev. Cell 50, 716-728.e6 (2019).
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