Strain Information
| Name | JH3201 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | fbf-2(ax2057[fbf-2::GFP]) II. |
| Description | Maintain at 20-25C. ax2057 was produced by mutation of the sgRNA site and insertion of GFP cDNA at the C-terminus of fbf-2. Insertion of GFP cDNA (from pCM1.53, no ATG/no STOP) at the C-terminus of fbf-2: ATCATCGCCGTGACTACCA(GFP) between II:6089145...6089165. sgRNA site was mutated to avoid Cas9 re-cutting. Reference: Paix A, et al. Genetics. 2014 Sep 23. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Alexandre Paix |
| Laboratory | JH |
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