Strain Information
| Name | JDW834 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | unc-119(kst33) col-97(wrd346[col-97::internal mNG::3xFLAG + unc-119(+)]) III. |
| Description | Superficially wild type. Modular linker::mNeonGreen::3xFLAG::linker tag inserted in the endogenous col-97 locus by CRISPR to produce a translational fusion inserted internally near the C-terminus (after amino acid 288). Allele obtained using plasmid-based unc-119 selection. Injection of a Cre recombinase plasmid failed to excise the loxP-flanked unc-119(+) cassette for unknown reasons. The insert was verified to be correct through Sanger sequencing. Knock-in is linked to the temperature-sensitive unc-119(kst33) allele. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Jordan Ward |
| Laboratory | JDW |
| Reference | Manuscript in preparation. |
Sign in
or
register an account if you want to order this strain.