Strain Information
| Name | IT1187 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | unc-119(ed3) III; kpIs100. |
| Description | kpIs100 [pie-1p::Ub(G76V)::GFP::H2B::drp-1 3' UTR + unc-119(+)]. [NOTE: (4/4/2025): Strain segregates Rol, and non-Rol, possibly due to a background dpy-10 mutation; both express GFP as expected. It was recommended that non-Rollers be picked when maintaining the strain.] Expresses Ubiquitin::GFP fusion protein in the germ line. The last amino acid of ubiquitin is mutated to valine (G76V), which prevents the cleavage of ubiquitin from GFP by the deubiqutinating enzymes and renders the GFP constitutively targeted for degradation by the proteasome. The strain can be used to assay proteasome activity in the germ line. Reference: Kumar GA & Subramaniam K. Development. 2018 Mar 29;145(7):dev163949. PMID: 29540500 |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Ganga Anil Kumar |
| Laboratory | IT |
| Reference | PUF-8 facilitates homologous chromosome pairing by promoting proteasome activity during meiotic entry in C. elegans G. A. Kumar and K. Subramaniam Development 2018 Vol. 145 Issue 7 Accession Number: 29540500 DOI: 10.1242/dev.163949 |
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