Laboratory Information

NameIT View on WormBase
Allele designationkp
HeadKuppuswamy Subramaniam
InstitutionIndian Institute of Technology, Madras, India
Address Indian Institute of Technology-Madras
Room#113, Department of Biotechnology
Chennai 600036
India
Website https://biotech.iitm.ac.in/faculty/subramaniam-k/
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
IT1187 unc-119(ed3) III; kpIs100. C. elegans kpIs100 [pie-1p::Ub(G76V)::GFP::H2B::drp-1 3' UTR + unc-119(+)]. Expresses Ubiquitin::GFP fusion protein in the germ line. The last amino acid of ubiquitin is mutated to valine (G76V), which prevents the cleavage of ubiquitin from GFP by the deubiqutinating enzymes and renders the GFP constitutively targeted for degradation by the proteasome. The strain can be used to assay proteasome activity in the germ line. Reference: Kumar GA & Subramaniam K. Development. 2018 Mar 29;145(7):dev163949. PMID: 29540500
IT540 gap-3(kp1) I; puf-8(zh17) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are wild type and segregate wild type heterozygotes, paralyzed DpyUncs, and Uncs with germline tumors. Pick WT and check segregation of progeny to maintain. Reference: Vaid S, et al. Development. 2013 Apr;140(8):1645-54.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
kp1 Allele substitution nonsense