Strain Information
| Name | IH36 View On Wormbase |
|---|---|
| Species | C.elegans |
| Genotype | rsf-1(tm5002) |
| Description | C.elegans rsf-1, the homologue of Drosophila dRASSF and mammalian RASSF genes (RASSF1-RASSF6), is deleted. About 14% rsf-1 mutants exhibit defects in embryonal morphogenesis and the actin disorganization. The combinatorial synthetic lethal analysis demonstrates that the lethality is enhanced to more than 80% in rsf-1 mutants with the WASP-family verprolin homologous protein complex-related gene depletions and corroborates the implication of RSF-1 in the regulation of actin cytoskeleton. In rsf-1 mutants, the structures of muscle actin are preserved, but the swimming ability is impaired. Although we could not detect the direct physical interaction of LET-60 with RSF-1, rsf-1 mutants suppress the multivulva phenotype of the active let-60 mutants, suggesting that rsf-1 genetically interacts with the Ras signaling. |
| Mutagen | |
| Outcrossed | x6 |
| Made by | Hiroaki Iwasa |
| Laboratory | 2874 |
| Reference | Characterization of RSF-1, the Caenorhabditis elegans homolog of the Ras-association domain family protein 1. Iwasa H, Kuroyanagi H, Maimaiti S, Ikeda M, Nakagawa K, Hata Y. Exp Cell Res. 2013 Feb 1;319(3):1-11. doi: 10.1016/j.yexcr.2012.10.008. Epub 2012 Oct 26.　PMID: 23103556 * The original mutant was generated by Prof. Shohei Mitani (National Bioresource Project, Japan) and was outcrossed by Hiroaki Iwasa. |
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