Strain Information
Name | HML1012 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | cshIs140 II; ieSi58 IV. |
Description | cshIs140 [rps-28p::TIR1(F79G)::T2A::mCherry::his-11 + Cbr-unc-119(+)] II. ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. Ubiquitously expressed single copy, modified TIR1 allele, TIR1(F79G) that is compatible with 5-PH-IAA and can be used to deplete auxin-induced degradation-tagged (AID-tagged) proteins. Efficiently depletes target proteins at 1µM 5-Ph-IAA. Nuclear localized mCherry co-expression marker. |
Mutagen | No mutagen |
Outcrossed | x4 |
Made by | Natalia Stec |
Laboratory | HML |
Reference | BioRxiv |
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