Strain Information
| Name | HML1012 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | cshIs140 II; ieSi58 IV. |
| Description | cshIs140 [rps-28p::TIR1(F79G)::T2A::mCherry::his-11 + Cbr-unc-119(+)] II. ieSi58 [eft-3p::AID*::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. Ubiquitously expressed single copy, modified TIR1 allele, TIR1(F79G) that is compatible with 5-PH-IAA and can be used to deplete auxin-induced degradation-tagged (AID-tagged) proteins. Efficiently depletes target proteins at 1 µM 5-Ph-IAA. Nuclear localized mCherry co-expression marker. Reference: Hills-Muckey et al. Genetics. 2022 Feb 4;220(2):iyab174. doi: 10.1093/genetics/iyab174. PMID: 34739048. |
| Mutagen | |
| Outcrossed | x4 |
| Made by | Natalia Stec |
| Laboratory | HML |
| Reference | BioRxiv |
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