Laboratory Information

NameHML View on WormBase
Allele designationcsh
HeadChristopher Martin Hammell
InstitutionCold Spring Harbor Laboratory
Address One Bungtown Road
James 213
Cold Spring Harbor 11724
United States
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
HML1012 cshIs140 II; ieSi58 IV. C. elegans cshIs140 [rps-28p::TIR1(F79G)::T2A::mCherry::his-11 + Cbr-unc-119(+)] II. ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. Ubiquitously expressed single copy, modified TIR1 allele, TIR1(F79G) that is compatible with 5-PH-IAA and can be used to deplete auxin-induced degradation-tagged (AID-tagged) proteins. Efficiently depletes target proteins at 1µM 5-Ph-IAA. Nuclear localized mCherry co-expression marker.
HML1035 cshIs128 II; ieSi58 IV. C. elegans cshIs128 [rps-28p::TIR1::T2A::mCherry::his-11 + Cbr-unc-119(+)] II. ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. Companion strain to HML1012. Harbors conventional allele of TIR1 and Nuclear localized mCherry co-expression marker.
This laboratory hasn't submitted any alleles to the CGC.