Strain Information
Name | GR1373 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | eri-1(mg366) IV. |
Description | Temperature sensitive: sterile at 25C. Maintain at 15C. Him. Eri. Due to a direct repeat the exact site and sequence of the 23 bp eri-1(mg366) insertion is unclear, however, the insertion lies in exon 6 of T07A9 between nucleotide positions 35215 and 35204 of cosmid T07A9 and includes 23 or these 32 nucleotides tttatcgaaaaaaaaacaggcactttatcgaa. Primers to follow eri-1mg366: GATAAAACTTCGGAACATATGGGGC and ACTGATGGGTAAGGAATCGAAGACG. These primers will give a 170 bp product in N2 and a 193 bp product in eri-1(mg366). |
Mutagen | EMS |
Outcrossed | x5 |
Made by | Scott Kennedy |
Laboratory | GR |
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