Strain Information

Name GOU2042   View On Wormbase
Species C. elegans
Genotypeeff-1(cas618 [eff-1::gfp]) II.
DescriptionGFP inserted into the endogenous eff-1 gene at its C-terminus by Cas9-triggered homologous recombination. Green fluorescence enriched in V.a cell 20min before cell-cell fusion in larvae. Very weak fluorescence in hyp7 cell. Reference: Yang Y, et al. Dev Cell. 2017 Apr 10;41(1):107-120.
MutagenCRISPR/Cas9 homologous recombination
Outcrossedx3
Made byYihong Yang
Laboratory GOU
Reference Yang, Y., Zhang, Y.1, Li, W-J., Jiang, Y., Zhu, Z., Hu, H., Li, W., Wu, J-W., Wang, Z-X., Dong, M-Q., Huang, S. and Ou, G.(2017) Spectraplakin Induces Positive Feedback between Fusogens and the Actin Cytoskeleton to Promote Cell-Cell Fusion. Developmental Cell. 41(1): 107-120. e4
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