Strain Information

Name GLW77   View On Wormbase
Species C. elegans
Genotypejnk-1(utx59[mNG::3xFlag::jnk-1]) IV.
DescriptionN-terminal tag of JNK-1 via CRISPR/Cas9 knock-in of mNeonGreen at jnk-1 locus. Insertion verified by PCR and fluorescence. Left flank: 5' cagcaagttaaagtgtgtgatcctgtgcac 3'; Right flank: 5' ATGGAGGAACGATTATCCACAACATCATCG 3’; gRNA: gtgtgatcctgtgcacATGG; Cas9/sgRNA plasmid: pGLOW114a; mNG^SEC^3xFlag plasmid: pGLOW116; SEC insertion allele strain: GLW76.
Made byKennedy Current (Glow Worms '21)
Laboratory GLW
Reference N/A
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