Strain Information
| Name | ESC825 View On Wormbase |
|---|---|
| Species | C.elegans |
| Genotype | wrdSi23 rpoa-2(cse772[AID*::GFP::rpoa-2]) I; ieSi11 II; nucl-1(cse770[nucl-1::mKate2]) IV. |
| Description | wrdSi23 [eft-3p::TIR1:F2A:mTagBFP::tbb2 3' UTR:: loxP] I. ieSi11 [syp-3p::EmGFP::syp-3::syp-3 3'UTR + Cbr-unc-119(+)] II. Maintain at 16-20C. AID*::GFP tag inserted at N-terminus of endogenous rpoa-2 locus. GFP::SYP-3 expression is readily detected in spermatocytes and oocytes in the germline, and localizes to the interface between paired homologous chromosomes during most of meiotic prophase. mKate2 tag inserted into the C-terminus of endogenous nucl-1 locus. Nucleolar mKate2 expression. This strain can be used for auxin-inducible degradation (AID) of RPOA-2 in somatic tissues. Reference: Mejia-Trujillo R, et al. bioRxiv 2025.05.07.652530; doi: https://doi.org/10.1101/2025.05.07.652530. |
| Mutagen | Crispr/Cas9, crossing |
| Outcrossed | x3 |
| Made by | Qiuxia Zhao |
| Laboratory | ESC |
| Reference | Mejia-Trujillo, R., Zhao, Q., Abraham, F., Rahman, A., and Cenik, E.S. (2025). RNA Pol I activity is required for meiotic chromatin organization and the H3K4me3 gradient essential for oogenesis, independent of ribosome synthesis. Preprint at bioRxiv, https://doi.org/10.1101/2025.05.07.652530 https://doi.org/10.1101/2025.05.07.652530. |
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