Strain Information

Name EL694   View On Wormbase
Species C. elegans
Genotypepup-4(om140) II.
Descriptionpup-4/F43E2.1. Maintain at 15-20C. Homozygotes produce <1% sterile individuals at 25C after multiple generations. ~10% of fertile individuals contain one infertile gonad arm. om140 is a CRISPR-engineered internal deletion removing most of the pup-4 coding sequence. Reference: Kelley L, et al. Development. 2024 Oct 7;228(2):iyae120. doi: 10.1093/genetics/iyae120. PMID: 39067069.
MutagenCrispr/Cas9
Outcrossedx4
Made byLeanne Kelley
Laboratory EL
Reference Kelley, L.H., I.V. Caldas, M.T. Sullenberger, K.E. Yongblah, A.M. Niazi, A. Iyer, Y. Li, P.M. Tran, E. Valen, Y.H. Ahmed-Braimah, E.M. Maine (2024) Poly(U) polymerase activity in C. elegans regulates abundance and tailing of sRNA and mRNA. Genetics, doi:10.1093/genetics/iyae120.
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