Laboratory Information

NameEL View on WormBase
Allele designationom
HeadEleanor M Maine
InstitutionSyracuse University, Syracuse, NY
Address Syracuse University
Dept of Biology
107 College Place / LSC
Syracuse 13244
United States
Website http://biology.syr.edu/faculty/maine/maine_research_1.htm
Gene classes ego  iffb  rrf  sog 

Strains contributed by this laboratory

Strain Genotype Species Description
EL129 ego-3(om40) unc-76(e911) V/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate additional hets, Unc-76 om40 homozygotes and dead eggs. om40 animals have multiple germline defects.
EL186 dpy-19(e1259) ego-4(om30)/eT1 III; +/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Dpy (ts) Mel, Unc-36 and dead eggs. dpy-19 ego-4 progeny die as embryos.
EL187 dpy-19(e1259) ego-5(om31)/eT1 III; +/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Dpy (ts) Mel, Unc-36 and dead eggs.
EL301 lag-1(om13) IV. C. elegans Temperature sensitive; best grown at 15C. Lab and embryonic Mel phenotypes.
EL302 ego-1(om71) unc-29(e193)/hT2 [dpy-18(h662)] I; +/hT2 [bli-4(e937)] III. C. elegans Heterozygotes are WT and segregate WT, Sterile Uncs and Dpys. bli-4 is suppressed by dpy-18 in hT2 homozygotes-only see a very few DpyBli.
EL322 ego-2(om33) fer-6(om117) I; him-5(e1467) V. C. elegans om33 chromosome contains a linked fer-6 allele and a linked Mel. Mel probably not associated with om33, but they have not been separated. Best grown at 15C.
EL329 ego-1(om58)/hT2 [dpy-18(h662)] I; +/hT2 [bli-4(e937)] III. C. elegans Heterozgyotes are WT and segregate WT, Steriles and Dpys. The Steriles produce small, abnormal oocytes; some dead embryos are produced in late adulthood. bli-4 is suppressed by dpy-1 in hT2 homozygotes-only see a very few DpyBli. om58 previously called ego-6(om58).
EL391 ego-1(om84) unc-29(e193)/hT2 [dpy-18(h662)] I; +/hT2 [bli-4(e937)] III. C. elegans Heterozygotes are WT and segregate WT, mild Unc that are Sterile, and Dpys. bli-4 is suppressed by dpy-18 in hT2 homozygotes-only see a very few DpyBli.
EL476 rrf-2(ok210) I. C. elegans M01G12.12. External left primer: GAGTGGTGGGCAATTGAGTT. External right primer: CCACCCAAGATCTGGTCAGT. Internal left primer: TGTCAACTTGAGGATCGACG. Internal right primer: ATTCCTGCAATTGGTCAAGG. Internal WT amplicon: 2509 bp. Deletion size: 979 bp. Deletion left flank: ATTTTCAACGAAAGTTTTTCGGTTATTTCA. Deletion right flank: AGAGGAAAAAATACGGACAAGAAGAATAAT.
EL477 iffb-1(bc367)/sC1 [dpy-1(s2170)] III. C. elegans Heterozygotes are WT and segregate WT, Dpy sC1 homozygotes, and bc367 homozygotes which arrest as late L1/early L2 larvae (survive for a few days, then die).
EL69 unc-32(e189) glp-1(q231) III; sog-3(q294) IV. C. elegans Unc. Fertile with viable progeny at or below 20C. Glp-1 sterile at higher temperatures. No obvious visible phenotype associated with sog-3.
JK845 sog-10(q162) dpy-17(e164) III. C. elegans Dpy. Cold-sensitive Fog phenotype; incompletely penetrant, even at 12C. Low percentage of sterile hermaphrodites with an Ooc phenotype. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK948 unc-32(e189) glp-1(q231) III; sog-4(q304) V. C. elegans Unc. sog-4 suppresses glp-1 at or below 20C. Do not maintain above 20C. Some Glp dead embryos because suppression is incomplete. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK966 unc-32(e189) glp-1(q224) III; sog-5(q297) X. C. elegans Unc. sog-5 suppresses glp-1 at or below 20C. Stock cannot be maintain above 20C. Some dead Glp embryos on plates. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK967 ubr-5(q298) I; unc-32(e189) glp-1(q231) III. C. elegans Unc. Fertile with viable progeny at or below 20C. Glp-1 sterile at higher temperatures. No obvious visible phenotype associated with sog-1. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK968 unc-32(e189) glp-1(q231) III; sog-6(q306) IV. C. elegans Unc. Fertile with viable progeny at or below 20C. Glp-1 sterile at higher temperatures. No obvious visible phenotype associated with sog-6. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
om40 Allele
om30 Allele
om31 Allele
om13 Allele substitution
om71 Allele substitution
om33 Allele substitution
om117 Allele
om58 Allele substitution
om84 Allele insertion
om97 Allele substitution nonsense