Strain Information
| Name | EL658 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | smrc-1(om138) met-2(om142[3xflag::met-2])/qC1 [dpy-19(e1259) glp-1(q339)] nIs189 III. |
| Description | Pick wild-type GFP+ to maintain. Heterozygotes are wild-type GFP+ (pharynx), and segregate wild-type GFP+ heterozygotes, Dpy Sterile GFP+, and non-GFP smrc-1(om138) met-2(om142) homozygotes (reduced fertility, reduced embryonic viability and high incidence of male offspring). Defects are more prominent in M-Z- animals at 25C. 3xFlag tag inserted at N-terminus of endogenous met-2 locus using Crispr/Cas9. nIs189 [myo-2::GFP] integrated in or near qC1. No recombination seen between nIs189 and qC1; fails to complement all markers on qC1. Reference: Yang B, et al. PLoS Genet. 2019 Feb 22;15(2):e1007992. doi: 10.1371/journal.pgen.1007992. PMID: 30794539. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x2 |
| Made by | Bing Yang |
| Laboratory | EL |
| Reference | Yang, B., X. Xu, L. Russell, M.T. Sullenberger, J.L. Yanowitz, E.M. Maine (2019) A DNA repair protein and histone methyltransferase interact to promote genome stability in C. elegans. PloS Genetics 15(2): e1007992. |
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