Strain Information
| Name | EG9891 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | unc-119(ox819) III; W03F9.11(oxTi1121) V. |
| Description | oxTi1121 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + lox2272]) V. The Cas9 transgene was optimized for germline expression by including 4 large PATC-rich introns from smu-2. Inserted into W03F9.11. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https://doi.org/10.1101/2021.08.03.454883 |
| Mutagen | miniMos, Crispr/Cas9 |
| Made by | Matthew Schwartz |
| Laboratory | EG |
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