Strain Information
| Name | EG9881 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | unc-119(ox819) F53A2.9(oxTi1127) III. |
| Description | oxTi1127 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + myo-2p::2xNLS::cyOFP::let-858 3'UTR + lox2272] III. Inserted into F53A2.9. High Cas9 activity. Integrated Cas9 transgene linked to unc-119(ox819). Cas9 insertion marked with myo-2p::cyOFP (cyan-excitable Orange Fluorescent Protein), a long-Stokes-shift fluorescent protein that is spectrally separable from common green and red fluorophores. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https://doi.org/10.1101/2021.08.03.454883 |
| Mutagen | miniMos, Crispr/Cas9 |
| Made by | Matthew Schwartz |
| Laboratory | EG |
Sign in
or
register an account if you want to order this strain.