Strain Information
Name | DV3805 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | reSi7 I. |
Description | reSi7 [rgef-1p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Neuronal-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in neuronal nuclei. NOTE: PCR detection of reSi7 insert using the published primers has been reported to be defective. These primers designed by Sherlyn Wijaya and Claire Richardson to detect ttTi4338 (LG I) also work for reIs7: ttTi4338 (LG I) wrdSi23-F: cttcaaagaaatcgccgac wrdSi23-FP: AACAACGAGACCTACGTCG wrdSi23-R: Ctctaagatgtcggccac (wt ~300bp, mutant ~650bp). |
Mutagen | CRISPR/Cas9 homologous recombination |
Outcrossed | x0 |
Made by | Tam Duong |
Laboratory | DV |
Reference | in preparation |
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