Strain Information

Name DV3805   View On Wormbase
Species C. elegans
GenotypereSi7 I.
DescriptionreSi7 [rgef-1p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Neuronal-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in neuronal nuclei. NOTE: PCR detection of this insert using the published primers has been reported to be defective. Neuronal-specific expression of blue fluorescent protein is still observed and neuronal-specific depletion of AID-tagged proteins has been reported. As such, the construct is inferred to be functional but will need to be tracked in strain constructions via a different method.
MutagenCRISPR/Cas9 homologous recombination
Made byTam Duong
Laboratory DV
Reference in preparation
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