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| C. elegans
|bmdSi245 I; hda-1(bmd134[HDA-1::GFP::loxP]) V.
|bmdSi245 [^SEC^lin-29p::mKate2-STOP-STOP-DAMc1::VHH4GFP] I. Relatively slow growth compared to N2 animals. NanoDam toolkit will allows identification of direct genomic targets of TFs as well as chromatin modifiers. In this system, Dam methylase is fused with a binding reagent, an anti-GFP nanobody (vhhGFP4). Thus, genome-wide profiling can be achieved by combining cell type-specific Dam::vhhGFP4 fusion constructs with GFP knock-in alleles.
|We are going to have a pre-print describing a new DamID-based approach, called NanoDam-ID in C. elegans, for genome-wide profiling targets, collaborating with Dr. Callista Yee and Dr. Michalis Barkoulas. Mechanisms of this tool is described below (other reason).