Strain Information
| Name | DLW14 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | unc-5(lib1[myo-3p::GFP(-) + unc-119(+) + myo-2p::GFP(Mos1)]) IV; krIs14 V. |
| Description | krIs14 [hsp-16.48p::MosTransposase + lin-15(+) + unc-122p::GFP] V. Recessive Unc. unc-5(lib1) is a CRISPR/Cas9 engineered mutant carrying the Intersister/Intrachromatid Repair Assay (ICR Assay) cassette inserted into the endogenous unc-5 locus. Briefly, ICR assay cassette includes two tandem GFP cassettes: the upstream using the myo-3 (body wall) promoter with a truncated GFP coding sequence, and the down-stream using the myo-2 (pharynx) promoter with GFP coding sequence interrupted by a Mos1 Drosophila transposon. Excision of Mos1 yields a single DSB, which if repaired by intersister or intrachromatid recombination, then will yield GFP+ progeny. The krIs14 insertion carrying heat-shock inducible Mos1 transposase is marked with coelomocyte GFP expression. Reference: Toraason E, et al. Current Biology 2021. https://doi.org/10.1016/j.cub.2021.03.008 |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x3 |
| Made by | Marissa Glover |
| Laboratory | DLW |
| Reference | Toraason, E., Horacek, A., Clark, C., Glover, M.L., Adler, V.L., Premkumar, T., Salagean, A., Cole, F., and Libuda, D.E. Meiotic DNA break repair can utilize homolog-independent chromatid templates in C. elegans. Current Biology 2021. https://doi.org/10.1016/j.cub.2021.03.008 |
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