Strain Information
| Name | DG4454 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | npp-12(ok2424) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III) |
| Description | Homozygous ok2424 animal are viable and fertile, but will go sterile in successive generations. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2424 homozygotes (superficially wild-type with some sterility). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Derived from parental strain RB1874, originally provided to the CGC by the OMRF Knockout Group, part of the International C. elegans Gene Knockout Consortium. Paper_evidence WBPaper00041807 |
| Mutagen | No mutagen |
| Outcrossed | x1 |
| Made by | Gabriela Huelgas Morales |
| Laboratory | DG |
| Reference | Deletion Mutant Consortium, T., Barstead, R. J., Moulder, G. L., Cobb, B., Frazee, S., Henthorn, D., Holmes, J., Jerebie, D., Landsdale, M., Osborne, J. D., Pritchett, C., Robertson, J., Rummage, J., Stokes, E., Vishwanathan, M., Mitani, S., Gengyo-Ando, K., Funatsu, O., Hori, S., Imae, R., Kage-Nakadai, E., Kobuna, H., Machiyama, E., Motohashi, T., Otori, M., Suehiro, Y., Yoshina, S., Moerman, D. G., Edgley, M. L., . . . Zapf, R. (2012). large-scale screening for targeted knockouts in the Caeno |
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