Laboratory Information

NameDG View on WormBase
Allele designationtn
HeadDavid I Greenstein
InstitutionUniversity of Minnesota, Minneapolis, MN
Address 4-208 MCB
420 Washington Avenue, SE
Minneapolis 55455
United States
Website http://www.gcd.med.umn.edu/html/faculty%20pages/greenstein.html
Gene classes looc  msd  sacy  std  lfor 

Strains contributed by this laboratory

Strain Genotype Species Description
BS14 gld-1(q266)/nDf24 I. C. elegans Heterozygotes are fertile and grow more slowly than gld-1(q266) homozygotes. gld-1(q266) homozygotes are sterile and produce small abnormal oocytes. nDf24 homozygotes are dead.
BS3164 unc-32(e189) glp-1(ar202) III. C. elegans Temperature sensitive. Maintain at 15 degrees. Germline tumor formation at 25 degrees. Proximal proliferations defects. Reference: Pepper et al. (2003) Genetics 163(1):115-32.
BS3392 gld-2(q497) gld-1(q485)/hT2 [dpy-18(h662)] I; unc-32(e189) glp-1(q175)/hT2 [bli-4(e937)] III. C. elegans Heterozygotes are wild-type and segregate WT heterozygotes, Unc (gld-2 gld-1; unc-32 glp-1 homozygotes), and Dpy (hT2 homozygotes; the bli-4 mutation is suppressed by dpy-18). Check Unc-32 animals for tumors to confirm presence of glp-1 in the line. glp-1(q175) is nonsense R191 > stop (opal).
BS553 fog-2(oz40) V. C. elegans Male/female strain. Maintain by mating.
CB4855 C. elegans wild isolate. C. elegans NOTE: Whole-genome analysis indicates that this stock is genotypically CB4858. Users interested in this strain are encouraged to obtain a verified CB4855-derivied strain. To obtain ECA247, a sequenced isolate of this wild strain, please visit the C. elegans Natural Diversity Resource at www.elegansvariation.org. Isolated from compost in Palo Alto, CA in 1982(?). Wild type (plg-1(e2001)). Low copy Tc1, pattern VI. Caenorhabditis elegans wild isolate CB subclone of Sta-5 (Tc1 pattern VI). Original stock isolated by T. Doniach.
CV138 sgo-1(tm2443) IV. C. elegans tm2443 is a 204 bp deletion + 7 bp insertion in 21762/21763-TTTTCTC-21966/21967. A low penetrance (1/25) of chromosome bridges is observed at anaphase I. Reference: de Carvalho et al., Genes Dev 22, 2869-2885.
CV6 lab-1(tm1791) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Heterozygotes are wild-type GFP+. Homozygotes (tm1791/tm1791) are 4% Him with 22% embryonic lethality. Maintain by picking GFP+. Reference: de Carvalho et al., Genes Dev 22, 2869-2885.
CZ2274 efn-4(bx80) efn-2(ev658) IV; efn-3(ev696) X. C. elegans bx80 was previously called mab-26(bx80): Extensive ray fusion involving all 9 rays; Larva have Vab phenotype with decreasing expressivity in adult; Hermaphrodites have swollen tail and anus. Vab, embryonic ventral enclosure defects, male ray fusions. Slow growth.
CZ2611 vab-2(ju1) efn-2(ev658) IV; efn-3(ev696) X. C. elegans vab-2(ju1) has embryonic lethality (12%) and notched heads (about 40%). vab-2(ju1) is considered a null allele (W30opal), and was previously called efn-1. Vab, embryonic ventral enclosure defects, male ray fusions.
DG1255 unc-53(e404) sqt-1(e1350) II. C. elegans e1350 is a recessive Dpy, dominant Roller. Unc.
DG1575 tnIs6. C. elegans tnIs6 [lim-7::GFP + rol-6(su1006)]. Rollers. lim-7::GFP is expressed in sheath cells (see Hall et al., 1999, Developmental Biology 212: 101-123. Insertion site not mapped.
DG1576 tnIs5. C. elegans tnIs5 [lim-7::GFP + rol-6(su1006)]. Rollers. lim-7::GFP is expressed in sheath cells (see Hall et al., 1999, Developmental Biology 212: 101-123. Insertion site not mapped.
DG1604 fog-2(q71) V; ceh-18(mg57) X. C. elegans Maintain by mating females with males.
DG1612 vab-1(dx31)/mIn1 [dpy-10(e128) mIs14] II; fog-2(q71) V. C. elegans Maintain by mating GFP+ females with GFP+ males.
DG1650 vab-1(dx31)/mIn1 [dpy-10(e128) mIs14] II; fog-2(q71) V; ceh-18(mg57) X. C. elegans Maintain by mating GFP+ females with GFP+ males.
DG1701 cgh-1(tn691) III. C. elegans Semi-dominant temperature-sensitive embryonic lethal. Maintain at 15C.
DG1770 cgh-1(ok492) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans C07H6.5. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok492 homozygotes (sterile adult, tends to explode at vulva). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GGCAGCTCGAAAATATTGCC. External right primer: GGAAAACCGCAAGGATGGTGG. Internal left primer: TCACGGAGCTAGATGTGACG. Internal right primer: CGTCAAAAAGAACCCGATGT. Internal WT amplicon: 3095 bp. Deletion size: 1043 bp. Deletion left flank: GAGAACATACACAATCTGGACGAGATCACT. Deletion right flank: CCTGGGGTGGCGATGACCAAGTGAACCGTT. This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. URL: http://www.celeganskoconsortium.omrf.org.
DG1856 goa-1(sa734) I. C. elegans Recessive, early stop mutation within the coding sequence (C to T substitution in aa52) makes sa734 a likely null allele. May grow slightly better at 15C. Hyperactive, lays early stage eggs, increased amplitude of locomotort wave-form. Suppresses the lethargy and egg-laying defects of unc-43(n498). Reverses direction of locomotion more frequently than WT.
DG1867 glp-1(tn777) III. C. elegans Temperature sensitive. Maintain at 15C.
DG2102 unc-119(ed3) III; tnIs13 V. C. elegans tnIs13 [pie-1p::vab-1::GFP + unc-119(+)].
DG2160 tnIs13 ltIs44 V. C. elegans tnIs13 [pie-1p::vab-1::GFP + unc-119(+)]. ltIs44 [pie-1p::mCherry::PH(PLC1delta1) + unc-119(+)].
DG2179 tub-1(nr2044) II. C. elegans
DG2189 fog-3(q443) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); tnIs13 ltIs44 V. C. elegans tnIs13 [pie-1p::vab-1::GFP + unc-119(+)]. ltIs44 [pie-1p::mCherry::PH(PLC1delta1) + unc-119(+)]. Homozygous hT2[bli-4 let-? qIs48] are inviable. Homozygous fog-3(q443) animals are females.
DG2190 fog-3(q443) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); tnIs13 V. C. elegans tnIs13 [pie-1p::vab-1::GFP + unc-119(+)]. Homozygous hT2[bli-4 let-? qIs48] are inviable. Homozygous fog-3(q443) animals are females.
DG2356 glp-1(tn777) III; fog-2(oz40) V. C. elegans Temperature sensitive. Maintain at 15C.
DG2389 glp-1(bn18) III. C. elegans Temperature sensitive. Maintain at 15C.
DG2474 glp-1(ar202) III; fog-2(oz40) V. C. elegans Temperature sensitive. Maintain at 15C.
DG2884 daf-2(e1370) III; fog-2(oz40) V. C. elegans Male-female strain. Maintain by mating at 15 C.
DG2913 egl-30(ad810) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ad810 homozygotes. Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain.
DG2941 spr-5(by134) I; acy-4(ok1806) V. C. elegans spr-5(by134) suppresses acy-4(ok1806) sterility. Reference: Kim S, et al. 2012 Genetics
DG3095 twk-1(tn1397) I; acy-4(ok1806) V. C. elegans twk-1(tn1397) suppresses acy-4(ok1806) sterility. acy-4(ok1806) suppresses twk-1(tn1397) adult-onset uncoordinated or paralyzed phenotype. Reference: Kim S, et al. 2012 Genetics
DG3226 deps-1(bn124) I. C. elegans MAINTAIN AT 20 degrees C! deps-1 mutants are temperature-sensitive maternal-effect sterile (>80% sterile at 24.5C) and deps-1 M-Z- animals tend to lay many dead eggs and fewer eggs than WT at lower temps.
DG3373 sacy-1(tn1385) I; acy-4(ok1806) V. C. elegans sacy-1(tn1385) suppresses acy-4(ok1806) sterility. Reference: Kim S, et al. 2012 Genetics
DG3391 acy-4(ok1806) V; tnEx37. C. elegans tnEx37 [acy-4(+) + sur-5::GFP]. Pick wild-type to maintain. Segregate GFP+ fertile animals and GFP- acy-4(ok1806) sterile animals. Reference: Kim S, et al. 2012 Genetics
DG3393 kin-1(ok338) I; tnEx109. C. elegans tnEx109 [kin-1(+) + sur-5::GFP]. Segregates GFP+ fertile animals and GFP- kin-1(ok338) animals (early larval lethal). Reference: Kim S, et al. 2012 Genetics
DG3408 twk-1(tn1397) I. C. elegans Adult-onset uncoordinated or paralyzed. DG3408 was segregated from DG3095 [twk-1(tn1397) I; acy-4(ok1806) V]. Reference: Kim S, et al. 2012 Genetics
DG3430 sacy-1(tn1385) I. C. elegans Superficial wild type. DG3430 was segregated from DG3373 [sacy-1(tn1385) I; acy-4(ok1806) V]. Reference: Kim S, et al. 2012 Genetics
DG3449 sacy-1(tn1385) I; fog-2(oz40) V. C. elegans sacy-1(tn1385) partially suppresses fog-2(oz40) self-sterility. Reference: Kim S, et al. 2012 Genetics
DG3485 sacy-1(tm5503) I; tnEx159. C. elegans tnEx159 [sacy-1p::GFP::sacy-1 + unc-119(+)]. Pick wild-type to maintain. Transgene rescues sacy-1(tm5503) sterility. Reference: Kim S, et al. 2012 Genetics
DG3492 sacy-1(tm5503) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Segregates WT GFP+ heterozygotes, non-GFP sacy-1(tm5503) sterile animals, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+. Reference: Kim S, et al. 2012 Genetics
DG3614 inx-8(tn1474) inx-9(ok1502) IV; tnEx195. C. elegans tnEx195 [inx-8(+) + inx-9(+) + sur-5::GFP]. Pick GFP+ animals to maintain. Animals carrying tnEx195 are wild-type; animals that have lost the array are sterile and produce few germ cells. Reference: Starich TA, Hall DH, Greenstein D. Genetics. 2014 Nov;198(3):1127-53.
DG3784 lin-41(tn1487) I. C. elegans Temperature-sensitive sterile. Maintain at 15C. Reference: Spike CA, et al. Genetics. 2014 Sep 26. pii: genetics.114.168831.
DG3887 inx-21(tn1540) I/hT2[bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Homozygous sterile allele balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP sterile homozygotes (inx-21(tn1540) homozygous animals are sterile, producing few germ cells). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Starich TA, Hall DH, Greenstein D. Genetics. 2014 Nov;198(3):1127-53.
DG3913 lin-41(tn1541[GFP::tev::s::lin-41]) I. C. elegans lin-41(tn1541[GFP::tev::s::lin-41]) I. May be maintained under normal conditions.
DG3922 tiar-1(tn1545[tiar-1::s::tev::GFP]) II. C. elegans tiar-1(tn1545[tiar-1::s::tev::GFP]) II. Reference: Huelgas-Morales G., et al. G3 (Bethesda). 2016 Apr; 6(4): 1031-1047.
DG3929 tiar-1(tn1543[loxP::Cbr-unc-119(+)::loxP]) II. C. elegans tiar-1(tn1543[loxP::Cbr-unc-119(+)::loxP]) II. Reference: Huelgas-Morales G., et al. G3 (Bethesda). 2016 Apr 7;6(4):1031-47.
DG3941 spr-5(by134) I; acy-4(ok1806) V. C. elegans spr-5(by134) suppresses acy-4(ok1806) sterility. Reference: Kim S, et al. 2012 Genetics
DG4105 lfor-1(tn1652) lfor-2(tn1653) II. C. elegans Apparently wild type
DG4153 pod-2(tn1691) II; tnEx212. C. elegans tnEx212 [pod-2(+) + sur-5::GFP]. Pick GFP+ animals to maintain. sur-5::gfp(+) animals are wild type and segregate GFP(+) wild-type animals and GFP(-) pod-2(tn1691) dead embryos. tn1691 deletes ~15 kb within pod-2, including most of Exon 2 through to and including the stop codon (but not the polyA site). Reference: Starich TA, et al. eLife 2020;9:e58619 DOI: 10.7554/eLife.58619 PMID: 32735213
DG4158 spn-4(tn1699[spn-4::gfp::3xflag]) V. C. elegans Apparently wild type
DG4190 cdc-25.3(tn1712[gfp::3xflag::cdc-25.3]) III. C. elegans Superficially wild type
DG4213 meg-1(tn1724[gfp::3xflag::meg-1]) X. C. elegans Superficially wild type
DG4215 puf-5(tn1726[gfp::3xflag::puf-5]) II. C. elegans Superficially wild type
DG4218 cpg-1(tn1728[mng::3xflag::cpg-1]) III. C. elegans Superficially wild type
DG4222 pos-1(tn1730[gfp::3xflag::pos-1]) V. C. elegans Superficially wild type
DG4228 orc-1(tn1732[mNeonGreen::3xflag::orc-1]) III. C. elegans Superficially wild type
DG4230 gla-3a(tn1734[gfp::3xflag::gla-3a]) I. C. elegans Superficially wild type
DG4233 pqn-59(tn1737[gfp::3xflag::pqn-59]) I. C. elegans Superficially wild type
DG4269 mex-3(tn1753[gfp::3xflag::mex-3]) I. C. elegans Superficially wild type
DG4329 fasn-1(tn1762) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III) C. elegans Homozygous lethal deletion balanced by bli-4- and GFP-marked translocation. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP heterozygotes, arrested hT2 aneuploids, and non-GFP tn1762 homozygotes (dead embryos and L1 larvae). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick wild-type GFP and check for correct segregation of progeny to maintain. tn1762 is a ~9.2 kb deletion within fasn-1, including Exon 2 thru to 57 nt preceding stop codon. Reference: Starich TA, et al. eLife 2020;9:e58619 DOI: 10.7554/eLife.58619 PMID: 32735213
DG4384 hmgr-1(tm4386) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I; III). C. elegans Heterozygotes are WT, GFP+ in the pharynx and segregate WT (GFP+ in the pharynx), dead eggs (homozygotes for hT2) and hmgr-1(tm4386) homozygotes, which die as young larvae (L1/L2). Maintain by picking GFP+ worms and checking for correct segregation, since the hT2 balancer is lost at low frequencies. hmgr-1(tm4386) can be maintained as homozygous fertile hermaphrodites on 20 mM mevalonolactone. Reference: P. Ranji, M. Rauthan, C. Pitot and M. Pilon, 2014. PloS ONE 9(6): e100033.
DG4392 cyb-3(tn1755[gfp::3xflag::cyb-3]) V. C. elegans Although this strain is maintainable as a homozygote it produces many dead embryos (~80%) and has a low viable brood size (~24 ± 18). Thus, the GFP tag compromises CYB-3 function.
DG4454 npp-12(ok2424) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III) C. elegans Homozygous ok2424 animal are viable and fertile, but will go sterile in successive generations. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2424 homozygotes (superficially wild-type with some sterility). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Derived from parental strain RB1874, originally provided to the CGC by the OMRF Knockout Group, part of the International C. elegans Gene Knockout Consortium. Paper_evidence WBPaper00041807
DG4569 cyb-1(tn1806[cyb-1::gfp::tev::3xflag]) IV. C. elegans Viable and fertile, grows and moves well. No apparent abnormalities yet noted. Reference: Spike CA, et al. Genetics. 2022 May 5;221(1):iyac051. doi: 10.1093/genetics/iyac051. PMID: 35377419
DG627 emb-30(tn377) III. C. elegans Temperature sensitive. Maintain at 15C. Male mating stock.
DG695 +/hT2 [dpy-18(h662)] I; unc-36(e251) evl-8(ar102)/hT2 [bli-4(e937)] III. C. elegans Heterozygotes are WT and segregate WT, Uncs with an everted Vulva, Dpys and dead eggs.
DG696 +/hT2 [dpy-18(h662)] I; unc-36(e251) evl-19(ar98)/hT2 [bli-4(e937)] III. C. elegans Heterozygotes are WT and segregate WT, Uncs with an everted Vulva, Dpys and dead eggs.
DG728 sma-2(e502) emb-30(tn377) ced-7(n1892) unc-69(e587) III. C. elegans Temperature sensitive emb-30 allele. Maintain at 15C. Small. Unc. Cell death abnormal.
DG753 emb-30(tn474) III. C. elegans Allele viable at all temperatures (15-25C).
DG769 unc-32(e189) emb-30(tn476) III/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36.
DG783 unc-32(e189) emb-30(tn478) III/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36.
DG784 unc-32(e189) emb-30(tn477) III/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Unc-32 maternal effect lethals (Mel) and Unc-36.
DG786 unc-32(e189) emb-30(tn480) III/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36.
DG796 sma-2(e502) tnDf2/sma-2(e502) ced-7(n1892) unc-69(e587) III. C. elegans Heterozygotes are SmaCed and segregate SmaCed, SmaCedUnc and dead eggs. Maintain by picking SmaCed. tnDf2 is not transmitted well by males (i.e. tnDf2/+ males have a low mating efficiency).
DG800 unc-32(e189) tnDf2 III/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. tnDf2 is not transmitted well by males (i.e. tnDf2/+ males have a low mating efficiency).
DG801 unc-32(e189) tnDf2/sma-2(e502) ced-7(n1892) unc-69(e587) III. C. elegans Heterozygotes are Ced and segregate Ced, dead eggs and SmaCedUncs. Maintain by picking Ced. tnDf2 is not transmitted well by males (i.e. tnDf2/+ males have a low mating efficiency).
DG815 unc-32(e189) emb-30(tn475)/unc-36(e251) III. C. elegans Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36.
DG832 emb-30(tn475)/eT1 III; unc-46(e177) mdf-1(gk2)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-46 Steriles, and Unc-36 (eT1 homozygotes). Maintain by picking WT.
DG844 emb-30(tn377) III; unc-46(e177) mdf-1(gk2) V. C. elegans emb-30(tn377ts) conditionally suppresses mdf-1(gk2). Maintain at 15C.
EG4348 C. elegans wild isolate. C. elegans Utah natural isolate carrying peel-1(qq99) I. EG4348 was collected by M. Ailion from Salt Lake City, UT. qq99 designates the naturally occurring nonsense mutation in peel-1. Reference: Seidel HS et al. PLoS Biol. 2011 Jul;9(7):e1001115.
EG5897 oxSi120 II; unc-119(ed3) III. C. elegans oxSi120 [peel-1p::tagRFP::msp-142 3'UTR + unc-119(+)]. Reference: Batchelder EL, et al. Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11429-34.
EH487 inx-3(lw68) X; lwEx27. C. elegans lwEx27 [inx-3::GFP]. inx-3(lw68) embryos display a wide range of developmental defects. All L1s that manage to hatch have a Pun (pharynx unattached) phenotype. inx-3::GFP transgene rescues lw68 to wild-type. Pick L2s or later to maintain. Reference: Starich, et al., 2003. Dev. Biol. 256:403.
GC833 glp-1(ar202) III. C. elegans Temperature sensitive. Maintain at 15C.
JUP1 oxSi120 II; him-8(e1489) IV. C. elegans oxSi120 [peel-1p::tagRFP::MSP-142 3'utr+ unc-119(+)]. Him. Derived from EG5897 and CB1489; not known if is unc-119(ed3) is still present in background. Reference: Batchelder EL, et al. Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11429-34.
KK1105 lgl-1(tm2616) X. C. elegans
OD70 unc-119(ed3) III; ltIs44 V. C. elegans ltIs44 [pie-1p::mCherry::PH(PLC1delta1) + unc-119(+)].
OK559 egrh-1(tm1736) X. C. elegans Homozygous viable.
QX1409 qqIR7 (I: peel-1(qq99), EG4348>N2); ttTi5605 II; unc-119(ed3) III. C. elegans Nonsense allele of peel-1 carried in Utah isolate EG4348 crossed into N2 Bristol background. Reference: Seidel HS et al. PLoS Biol. 2011 Jul;9(7):e1001115.
SL438 spe-9(eb19) I; him-5(e1490) V; ebEx126. C. elegans ebEx126 [YAC Y47H9 [spe-9(+)] + rol-6(su1006)]. Pick Rollers to maintain. eb19 is a spe-9 non-conditional mutant.
SS747 bnIs1. C. elegans bnIs1[pie-1::GFP::pgl-1 + unc-119(+)]. GFP is brightest at 25C and the strain should be grown at that temperature. Routinely pick bright GFP+ worms to maintain. Because the transgene can become silenced, you should check the worms for GFP expression and freeze as soon as possible.
ZB1748 Y32H12A.8(tm422) III. C. elegans
ZB2774 Y32H12A.8(tm419) III. C. elegans

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
tn691 Allele substitution
tn777 Allele substitution
tn1397 Allele substitution nonsense
tn1385 Allele substitution
tn1474 Allele deletion
tn1487 substitution
tn1540 Allele deletion
tn377 Allele substitution
tn474 Allele substitution nonsense
tn476 Allele substitution nonsense
tn478 Allele substitution
tn477 Allele substitution nonsense
tn480 Allele substitution nonsense
tn475 Allele substitution nonsense
tn1231 Allele substitution splice_site