Laboratory Information
| Name | DG View on WormBase |
|---|---|
| Allele designation | tn |
| Head | David I Greenstein |
| Institution | University of Minnesota, Minneapolis, MN |
| Address | 4-208 MCB 420 Washington Avenue, SE Minneapolis 55455 United States |
| Website | http://www.gcd.med.umn.edu/html/faculty%20pages/greenstein.html |
| Gene classes | looc msd sacy std lfor |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| AG400 | fasn-1(av138[fasn-1::gfp]) I. | C. elegans | Homozygous viable, gfp expression in intestine, hypodermis, developing vulva, somatic gonad. Reference: Starich et al. eLife 2020;9:e58619. DOI: https://doi.org/10.7554/eLife.58619 |
| BS14 | gld-1(q266)/nDf24 I. | C. elegans | Heterozygotes are fertile and grow more slowly than gld-1(q266) homozygotes. gld-1(q266) homozygotes are sterile and produce small abnormal oocytes. nDf24 homozygotes are dead. |
| BS3164 | unc-32(e189) glp-1(ar202) III. | C. elegans | Temperature sensitive. Maintain at 15 degrees. Germline tumor formation at 25 degrees. Proximal proliferations defects. Reference: Pepper et al. (2003) Genetics 163(1):115-32. |
| BS3392 | gld-2(q497) gld-1(q485)/hT2 [dpy-18(h662)] I; unc-32(e189) glp-1(q175)/hT2 [bli-4(e937)] III. | C. elegans | Heterozygotes are wild-type and segregate WT heterozygotes, Unc (gld-2 gld-1; unc-32 glp-1 homozygotes), and Dpy (hT2 homozygotes; the bli-4 mutation is suppressed by dpy-18). Check Unc-32 animals for tumors to confirm presence of glp-1 in the line. glp-1(q175) is nonsense R191 > stop (opal). |
| BS553 | fog-2(oz40) V. | C. elegans | Male/female strain. Maintain by mating. |
| CB4855 | C. elegans wild isolate. | C. elegans | NOTE: Whole-genome analysis indicates that this stock is genotypically CB4858. Users interested in this strain are encouraged to obtain a verified CB4855-derivied strain. To obtain ECA247, a sequenced isolate of this wild strain, please visit the C. elegans Natural Diversity Resource at www.elegansvariation.org. Isolated from compost in Palo Alto, CA in 1982(?). Wild type (plg-1(e2001)). Low copy Tc1, pattern VI. Caenorhabditis elegans wild isolate CB subclone of Sta-5 (Tc1 pattern VI). Original stock isolated by T. Doniach. |
| CV138 | sgo-1(tm2443) IV. | C. elegans | tm2443 is a 204 bp deletion + 7 bp insertion in 21762/21763-TTTTCTC-21966/21967. A low penetrance (1/25) of chromosome bridges is observed at anaphase I. Reference: de Carvalho et al., Genes Dev 22, 2869-2885. |
| CV6 | lab-1(tm1791) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Heterozygotes are wild-type GFP+. Homozygotes (tm1791/tm1791) are 4% Him with 22% embryonic lethality. Maintain by picking GFP+. Reference: de Carvalho et al., Genes Dev 22, 2869-2885. |
| CZ2274 | efn-4(bx80) efn-2(ev658) IV; efn-3(ev696) X. | C. elegans | bx80 was previously called mab-26(bx80): Extensive ray fusion involving all 9 rays; Larva have Vab phenotype with decreasing expressivity in adult; Hermaphrodites have swollen tail and anus. Vab, embryonic ventral enclosure defects, male ray fusions. Slow growth. |
| CZ2611 | vab-2(ju1) efn-2(ev658) IV; efn-3(ev696) X. | C. elegans | vab-2(ju1) has embryonic lethality (12%) and notched heads (about 40%). vab-2(ju1) is considered a null allele (W30opal), and was previously called efn-1. Vab, embryonic ventral enclosure defects, male ray fusions. |
| DG1255 | unc-53(e404) sqt-1(e1350) II. | C. elegans | e1350 is a recessive Dpy, dominant Roller. Unc. |
| DG1575 | tnIs6. | C. elegans | tnIs6 [lim-7::GFP + rol-6(su1006)]. Rollers. lim-7::GFP is expressed in sheath cells (see Hall et al., 1999, Developmental Biology 212: 101-123. Insertion site not mapped. |
| DG1576 | tnIs5. | C. elegans | tnIs5 [lim-7::GFP + rol-6(su1006)]. Rollers. lim-7::GFP is expressed in sheath cells (see Hall et al., 1999, Developmental Biology 212: 101-123. Insertion site not mapped. |
| DG1604 | fog-2(q71) V; ceh-18(mg57) X. | C. elegans | Maintain by mating females with males. |
| DG1612 | vab-1(dx31)/mIn1 [dpy-10(e128) mIs14] II; fog-2(q71) V. | C. elegans | Maintain by mating GFP+ females with GFP+ males. |
| DG1650 | vab-1(dx31)/mIn1 [dpy-10(e128) mIs14] II; fog-2(q71) V; ceh-18(mg57) X. | C. elegans | Maintain by mating GFP+ females with GFP+ males. |
| DG1701 | cgh-1(tn691) III. | C. elegans | Semi-dominant temperature-sensitive embryonic lethal. Maintain at 15C. |
| DG1770 | cgh-1(ok492) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | C07H6.5. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok492 homozygotes (sterile adult, tends to explode at vulva). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GGCAGCTCGAAAATATTGCC. External right primer: GGAAAACCGCAAGGATGGTGG. Internal left primer: TCACGGAGCTAGATGTGACG. Internal right primer: CGTCAAAAAGAACCCGATGT. Internal WT amplicon: 3095 bp. Deletion size: 1043 bp. Deletion left flank: GAGAACATACACAATCTGGACGAGATCACT. Deletion right flank: CCTGGGGTGGCGATGACCAAGTGAACCGTT. This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. URL: http://www.celeganskoconsortium.omrf.org. |
| DG1856 | goa-1(sa734) I. | C. elegans | Recessive, early stop mutation within the coding sequence (C to T substitution in aa52) makes sa734 a likely null allele. May grow slightly better at 15C. Hyperactive, lays early stage eggs, increased amplitude of locomotory wave-form. Suppresses the lethargy and egg-laying defects of unc-43(n498). Reverses direction of locomotion more frequently than WT. |
| DG1867 | glp-1(tn777) III. | C. elegans | Temperature sensitive. Maintain at 15C. |
| DG2102 | unc-119(ed3) III; tnIs13 V. | C. elegans | tnIs13 [pie-1p::vab-1::GFP + unc-119(+)]. |
| DG2160 | tnIs13 ltIs44 V. | C. elegans | tnIs13 [pie-1p::vab-1::GFP + unc-119(+)]. ltIs44 [pie-1p::mCherry::PH(PLC1delta1) + unc-119(+)]. |
| DG2179 | tub-1(nr2044) II. | C. elegans | |
| DG2189 | fog-3(q443) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); tnIs13 ltIs44 V. | C. elegans | tnIs13 [pie-1p::vab-1::GFP + unc-119(+)]. ltIs44 [pie-1p::mCherry::PH(PLC1delta1) + unc-119(+)]. Homozygous hT2[bli-4 let-? qIs48] are inviable. Homozygous fog-3(q443) animals are females. |
| DG2190 | fog-3(q443) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); tnIs13 V. | C. elegans | tnIs13 [pie-1p::vab-1::GFP + unc-119(+)]. Homozygous hT2[bli-4 let-? qIs48] are inviable. Homozygous fog-3(q443) animals are females. |
| DG2356 | glp-1(tn777) III; fog-2(oz40) V. | C. elegans | Temperature sensitive. Maintain at 15C. |
| DG2389 | glp-1(bn18) III. | C. elegans | Temperature sensitive. Maintain at 15C. |
| DG2474 | glp-1(ar202) III; fog-2(oz40) V. | C. elegans | Temperature sensitive. Maintain at 15C. |
| DG2884 | daf-2(e1370) III; fog-2(oz40) V. | C. elegans | Male-female strain. Maintain by mating at 15 C. |
| DG2913 | egl-30(ad810) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ad810 homozygotes. Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. |
| DG2941 | spr-5(by134) I; acy-4(ok1806) V. | C. elegans | spr-5(by134) suppresses acy-4(ok1806) sterility. Reference: Kim S, et al. 2012 Genetics |
| DG3095 | twk-1(tn1397) I; acy-4(ok1806) V. | C. elegans | twk-1(tn1397) suppresses acy-4(ok1806) sterility. acy-4(ok1806) suppresses twk-1(tn1397) adult-onset uncoordinated or paralyzed phenotype. Reference: Kim S, et al. 2012 Genetics |
| DG3226 | deps-1(bn124) I. | C. elegans | MAINTAIN AT 20 degrees C! deps-1 mutants are temperature-sensitive maternal-effect sterile (>80% sterile at 24.5C) and deps-1 M-Z- animals tend to lay many dead eggs and fewer eggs than WT at lower temps. |
| DG3373 | sacy-1(tn1385) I; acy-4(ok1806) V. | C. elegans | sacy-1(tn1385) suppresses acy-4(ok1806) sterility. Reference: Kim S, et al. 2012 Genetics |
| DG3391 | acy-4(ok1806) V; tnEx37. | C. elegans | tnEx37 [acy-4(+) + sur-5::GFP]. Pick GFP+ animals to maintain. tnEx37 rescues acy-4(ok1806) sterility. Reference: Kim S, et al. 2012 Genetics |
| DG3393 | kin-1(ok338) I; tnEx109. | C. elegans | tnEx109 [kin-1(+) + sur-5::GFP]. Segregates GFP+ fertile animals and GFP- kin-1(ok338) animals (early larval lethal). Reference: Kim S, et al. 2012 Genetics |
| DG3408 | twk-1(tn1397) I. | C. elegans | Adult-onset uncoordinated or paralyzed. DG3408 was segregated from DG3095 [twk-1(tn1397) I; acy-4(ok1806) V]. Reference: Kim S, et al. 2012 Genetics |
| DG3430 | sacy-1(tn1385) I. | C. elegans | Superficial wild type. DG3430 was segregated from DG3373 [sacy-1(tn1385) I; acy-4(ok1806) V]. Reference: Kim S, et al. 2012 Genetics |
| DG3449 | sacy-1(tn1385) I; fog-2(oz40) V. | C. elegans | sacy-1(tn1385) partially suppresses fog-2(oz40) self-sterility. Reference: Kim S, et al. 2012 Genetics |
| DG3485 | sacy-1(tm5503) I; tnEx159. | C. elegans | tnEx159 [sacy-1p::GFP::sacy-1 + unc-119(+)]. Pick wild-type to maintain. Transgene rescues sacy-1(tm5503) sterility. Reference: Kim S, et al. 2012 Genetics |
| DG3492 | sacy-1(tm5503) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Segregates WT GFP+ heterozygotes, non-GFP sacy-1(tm5503) sterile animals, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+. Reference: Kim S, et al. 2012 Genetics |
| DG3614 | inx-8(tn1474) inx-9(ok1502) IV; tnEx195. | C. elegans | tnEx195 [inx-8(+) + inx-9(+) + sur-5::GFP]. Pick GFP+ animals to maintain. Animals carrying tnEx195 are wild-type; animals that have lost the array are sterile and produce few germ cells. Reference: Starich TA, Hall DH, Greenstein D. Genetics. 2014 Nov;198(3):1127-53. |
| DG3784 | lin-41(tn1487) I. | C. elegans | Temperature-sensitive sterile. Maintain at 15C. Reference: Spike CA, et al. Genetics. 2014 Sep 26. pii: genetics.114.168831. |
| DG3887 | inx-21(tn1540) I/hT2[bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Homozygous sterile allele balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP sterile homozygotes (inx-21(tn1540) homozygous animals are sterile, producing few germ cells). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Starich TA, Hall DH, Greenstein D. Genetics. 2014 Nov;198(3):1127-53. |
| DG3913 | lin-41(tn1541[GFP::tev::s::lin-41]) I. | C. elegans | lin-41(tn1541[GFP::tev::s::lin-41]) I. May be maintained under normal conditions. |
| DG3922 | tiar-1(tn1545[tiar-1::s::tev::GFP]) II. | C. elegans | tiar-1(tn1545[tiar-1::s::tev::GFP]) II. Reference: Huelgas-Morales G., et al. G3 (Bethesda). 2016 Apr; 6(4): 1031-1047. |
| DG3929 | tiar-1(tn1543[loxP::Cbr-unc-119(+)::loxP]) II. | C. elegans | tiar-1(tn1543[loxP::Cbr-unc-119(+)::loxP]) II. Reference: Huelgas-Morales G., et al. G3 (Bethesda). 2016 Apr 7;6(4):1031-47. |
| DG4105 | lfor-1(tn1652) lfor-2(tn1653) II. | C. elegans | Apparently wild type |
| DG4153 | pod-2(tn1691) II; tnEx212. | C. elegans | tnEx212 [pod-2(+) + sur-5::GFP]. Pick GFP+ animals to maintain. sur-5::gfp(+) animals are wild type and segregate GFP(+) wild-type animals and GFP(-) pod-2(tn1691) dead embryos. tn1691 deletes ~15 kb within pod-2, including most of Exon 2 through to and including the stop codon (but not the polyA site). Reference: Starich TA, et al. eLife 2020;9:e58619 DOI: 10.7554/eLife.58619 PMID: 32735213 |
| DG4158 | spn-4(tn1699[spn-4::gfp::3xflag]) V. | C. elegans | Apparently wild type |
| DG4190 | cdc-25.3(tn1712[gfp::3xflag::cdc-25.3]) III. | C. elegans | Superficially wild type |
| DG4213 | meg-1(tn1724[gfp::3xflag::meg-1]) X. | C. elegans | Superficially wild type |
| DG4215 | puf-5(tn1726[gfp::3xflag::puf-5]) II. | C. elegans | Superficially wild type |
| DG4218 | cpg-1(tn1728[mNG::3xflag::cpg-1]) III. | C. elegans | mNeonGreen and Flag tags inserted at 5' end of endogenous cpg-1 locus. Superficially wild type. |
| DG4222 | pos-1(tn1730[gfp::3xflag::pos-1]) V. | C. elegans | Superficially wild type |
| DG4228 | orc-1(tn1732[mNeonGreen::3xflag::orc-1]) III. | C. elegans | Superficially wild type |
| DG4230 | gla-3a(tn1734[gfp::3xflag::gla-3a]) I. | C. elegans | Superficially wild type |
| DG4233 | pqn-59(tn1737[gfp::3xflag::pqn-59]) I. | C. elegans | Superficially wild type |
| DG4269 | mex-3(tn1753[gfp::3xflag::mex-3]) I. | C. elegans | Superficially wild type |
| DG4324 | pod-2(tn1765[gfp::3xflag::pod-2]) II. | C. elegans | Homozygous viable, gfp expression in intestine, hypodermis, somatic gonad, excretory duct, CAN neuron. Reference: Starich et al. eLife 2020;9:e58619. DOI: https://doi.org/10.7554/eLife.58619 |
| DG4329 | fasn-1(tn1762) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III) | C. elegans | Homozygous lethal deletion balanced by bli-4- and GFP-marked translocation. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP heterozygotes, arrested hT2 aneuploids, and non-GFP tn1762 homozygotes (dead embryos and L1 larvae). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick wild-type GFP and check for correct segregation of progeny to maintain. tn1762 is a ~9.2 kb deletion within fasn-1, including Exon 2 thru to 57 nt preceding stop codon. Reference: Starich TA, et al. eLife 2020;9:e58619 DOI: 10.7554/eLife.58619 PMID: 32735213 |
| DG4384 | hmgr-1(tm4386) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I; III). | C. elegans | Heterozygotes are WT, GFP+ in the pharynx and segregate WT (GFP+ in the pharynx), dead eggs (homozygotes for hT2) and hmgr-1(tm4386) homozygotes, which die as young larvae (L1/L2). Maintain by picking GFP+ worms and checking for correct segregation, since the hT2 balancer is lost at low frequencies. hmgr-1(tm4386) can be maintained as homozygous fertile hermaphrodites on 20 mM mevalonolactone. Reference: P. Ranji, M. Rauthan, C. Pitot and M. Pilon, 2014. PloS ONE 9(6): e100033. |
| DG4392 | cyb-3(tn1755[gfp::3xflag::cyb-3]) V. | C. elegans | Although this strain is maintainable as a homozygote it produces many dead embryos (~80%) and has a low viable brood size (~24 ± 18). Thus, the GFP tag compromises CYB-3 function. |
| DG4454 | npp-12(ok2424) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III) | C. elegans | Homozygous ok2424 animal are viable and fertile, but will go sterile in successive generations. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2424 homozygotes (superficially wild-type with some sterility). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Derived from parental strain RB1874, originally provided to the CGC by the OMRF Knockout Group, part of the International C. elegans Gene Knockout Consortium. Paper_evidence WBPaper00041807 |
| DG4569 | cyb-1(tn1806[cyb-1::gfp::tev::3xflag]) IV. | C. elegans | Viable and fertile, grows and moves well. No apparent abnormalities yet noted. Reference: Spike CA, et al. Genetics. 2022 May 5;221(1):iyac051. doi: 10.1093/genetics/iyac051. PMID: 35377419 |
| DG627 | emb-30(tn377) III. | C. elegans | Temperature sensitive. Maintain at 15C. Male mating stock. |
| DG695 | +/hT2 [dpy-18(h662)] I; unc-36(e251) evl-8(ar102)/hT2 [bli-4(e937)] III. | C. elegans | Heterozygotes are WT and segregate WT, Uncs with an everted Vulva, Dpys and dead eggs. |
| DG696 | +/hT2 [dpy-18(h662)] I; unc-36(e251) evl-19(ar98)/hT2 [bli-4(e937)] III. | C. elegans | Heterozygotes are WT and segregate WT, Uncs with an everted Vulva, Dpys and dead eggs. |
| DG728 | sma-2(e502) emb-30(tn377) ced-7(n1892) unc-69(e587) III. | C. elegans | Temperature sensitive emb-30 allele. Maintain at 15C. Small. Unc. Cell death abnormal. |
| DG753 | emb-30(tn474) III. | C. elegans | Allele viable at all temperatures (15-25C). |
| DG769 | unc-32(e189) emb-30(tn476) III/eT1 (III;V). | C. elegans | Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36. |
| DG783 | unc-32(e189) emb-30(tn478) III/eT1 (III;V). | C. elegans | Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36. |
| DG784 | unc-32(e189) emb-30(tn477) III/eT1 (III;V). | C. elegans | Heterozygotes are WT and segregate WT, Unc-32 maternal effect lethals (Mel) and Unc-36. |
| DG786 | unc-32(e189) emb-30(tn480) III/eT1 (III;V). | C. elegans | Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36. |
| DG796 | sma-2(e502) tnDf2/sma-2(e502) ced-7(n1892) unc-69(e587) III. | C. elegans | Heterozygotes are SmaCed and segregate SmaCed, SmaCedUnc and dead eggs. Maintain by picking SmaCed. tnDf2 is not transmitted well by males (i.e. tnDf2/+ males have a low mating efficiency). |
| DG800 | unc-32(e189) tnDf2 III/eT1 (III;V). | C. elegans | Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. tnDf2 is not transmitted well by males (i.e. tnDf2/+ males have a low mating efficiency). |
| DG801 | unc-32(e189) tnDf2/sma-2(e502) ced-7(n1892) unc-69(e587) III. | C. elegans | Heterozygotes are Ced and segregate Ced, dead eggs and SmaCedUncs. Maintain by picking Ced. tnDf2 is not transmitted well by males (i.e. tnDf2/+ males have a low mating efficiency). |
| DG815 | unc-32(e189) emb-30(tn475)/unc-36(e251) III. | C. elegans | Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36. |
| DG832 | emb-30(tn475)/eT1 III; unc-46(e177) mdf-1(gk2)/eT1 V. | C. elegans | Heterozygotes are WT and segregate WT, Unc-46 Steriles, and Unc-36 (eT1 homozygotes). Maintain by picking WT. |
| DG844 | emb-30(tn377) III; unc-46(e177) mdf-1(gk2) V. | C. elegans | emb-30(tn377ts) conditionally suppresses mdf-1(gk2). Maintain at 15C. |
| EG4348 | C. elegans wild isolate. | C. elegans | Utah natural isolate carrying peel-1(qq99) I. EG4348 was collected by M. Ailion from Salt Lake City, UT. qq99 designates the naturally occurring nonsense mutation in peel-1. Reference: Seidel HS et al. PLoS Biol. 2011 Jul;9(7):e1001115. |
| EG5897 | oxSi120 II; unc-119(ed3) III. | C. elegans | oxSi120 [peel-1p::tagRFP::msp-142 3'UTR + unc-119(+)]. Reference: Batchelder EL, et al. Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11429-34. |
| EH487 | inx-3(lw68) X; lwEx27. | C. elegans | lwEx27 [inx-3::GFP]. inx-3(lw68) embryos display a wide range of developmental defects. All L1s that manage to hatch have a Pun (pharynx unattached) phenotype. inx-3::GFP transgene rescues lw68 to wild-type. Pick L2s or later to maintain. Reference: Starich, et al., 2003. Dev. Biol. 256:403. |
| GC833 | glp-1(ar202) III. | C. elegans | Temperature sensitive. Maintain at 15C. |
| JUP1 | oxSi120 II; him-8(e1489) IV. | C. elegans | oxSi120 [peel-1p::tagRFP::MSP-142 3'utr+ unc-119(+)]. Him. Derived from EG5897 and CB1489; not known if is unc-119(ed3) is still present in background. Reference: Batchelder EL, et al. Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11429-34. |
| KK1105 | lgl-1(tm2616) X. | C. elegans | |
| OD70 | unc-119(ed3) III; ltIs44 V. | C. elegans | ltIs44 [pie-1p::mCherry::PH(PLC1delta1) + unc-119(+)]. |
| OK559 | egrh-1(tm1736) X. | C. elegans | Homozygous viable. |
| QX1409 | qqIR7 (I: peel-1(qq99), EG4348>N2); ttTi5605 II; unc-119(ed3) III. | C. elegans | Nonsense allele of peel-1 carried in Utah isolate EG4348 crossed into N2 Bristol background. Reference: Seidel HS et al. PLoS Biol. 2011 Jul;9(7):e1001115. |
| SL438 | spe-9(eb19) I; him-5(e1490) V; ebEx126. | C. elegans | ebEx126 [YAC Y47H9 [spe-9(+)] + rol-6(su1006)]. Pick Rollers to maintain. eb19 is a spe-9 non-conditional mutant. |
| SS747 | bnIs1. | C. elegans | bnIs1[pie-1::GFP::pgl-1 + unc-119(+)]. GFP is brightest at 25C and the strain should be grown at that temperature. Routinely pick bright GFP+ worms to maintain. Because the transgene can become silenced, you should check the worms for GFP expression and freeze as soon as possible. |
| ZB1748 | Y32H12A.8(tm422) III. | C. elegans | |
| ZB2774 | Y32H12A.8(tm419) III. | C. elegans |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| tn691 | Allele | substitution | |
| tn777 | Allele | substitution | |
| tn1397 | Allele | substitution | nonsense |
| tn1385 | Allele | substitution | |
| tn1474 | Allele | deletion | |
| tn1487 | substitution | ||
| tn1540 | Allele | deletion | |
| tn377 | Allele | substitution | |
| tn474 | Allele | substitution | nonsense |
| tn476 | Allele | substitution | nonsense |
| tn478 | Allele | substitution | |
| tn477 | Allele | substitution | nonsense |
| tn480 | Allele | substitution | nonsense |
| tn475 | Allele | substitution | nonsense |
| tn1231 | Allele | substitution | splice_site |