Strain Information
| Name | DG4329 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | fasn-1(tn1762) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III) |
| Description | Homozygous lethal deletion balanced by bli-4- and GFP-marked translocation. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP heterozygotes, arrested hT2 aneuploids, and non-GFP tn1762 homozygotes (dead embryos and L1 larvae). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick wild-type GFP and check for correct segregation of progeny to maintain. tn1762 is a ~9.2 kb deletion within fasn-1, including Exon 2 thru to 57 nt preceding stop codon. Reference: Starich TA, et al. eLife 2020;9:e58619 DOI: 10.7554/eLife.58619 PMID: 32735213 |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x2 |
| Made by | Todd Starich |
| Laboratory | DG |
| Reference | Gap junctions deliver malonyl-CoA from soma to germline to support embryogenesis in Caenorhabditis elegans. Todd A Starich, Xiaofei Bai, and David Greenstein. eLife 2020;9:e58619 DOI: 10.7554/eLife.58619 |
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