Strain Information
| Name | DCR8374 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | pfk-1.1(ola458)/tmc30 x |
| Description | Allele ola458 is balanced with a marker strain that has a reversed X chromosome to prevent crossover events (FX30236). This line is heterozygous for the PFK-1.1 ko and the marker chromosome. FX30236 has a CRISPR'd red pharynx marker, a recessive long phenotype, and a weak recessive mec phenotype. Maintain strain by picking non-long, non-mec worms with the red pharynx marker. Progeny of hets that are homozygous for the marker have a red pharynx with about 150% longer body length (generally skinnier too) and are mildly mechano-insensitive; homozygous for the PFK-null allele are normal body length with no pharynx marker with the high embryo fatality phenotype. |
| Mutagen | |
| Outcrossed | x3 |
| Made by | Aaron Wolfe |
| Laboratory | DCR |
| Reference | A.D. Wolfe, J.N. Koberstein, C.B. Smith, M.L. Stewart, I.J. Gonzalez, M. Hammarlund, A.A. Hyman, P.J.S. Stork, R.H. Goodman, & D.A. Colón-Ramos, Local and dynamic regulation of neuronal glycolysis in vivo, Proc. Natl. Acad. Sci. U.S.A. 121 (3) e2314699121, https://doi.org/10.1073/pnas.2314699121 (2024). |
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