Strain Information
| Name | BFF68 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | mjIs134 II; hrde-1(pig6[degron::ha::hrde-1]) III. |
| Description | mjIs134 [mex-5p::GFP::(Gly)5Ala::his-58::tbb-2 3'UTR + Cbr-unc-119(+)] II. N-terminal Auxin-Inducible Degron and HA tag inserted into the endogenous hrde-1 locus. gfp fluorescence in germline (mex-5::gfp). Control strain for BFF69; does not carry TIR1 transgene necessary for the degradation. hrde-1(pig6) crRNA sequence: CAUAAUUUUGUCGAGCAAGU. Reference: Toker IA, et al. Dev Cell. 2022 Feb 7;57(3):298-309.e9. PMID: 35134343. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Olga Antonova |
| Laboratory | BFF |
| Reference | Toker, I. A., Lev, I., Mor, Y., Gurevich, Y., Fisher, D., Houri-Zeevi, L., ... & Rechavi, O. (2020). Transgenerational Regulation of Sexual Attractiveness in C. elegans Nematodes. bioRxiv. |
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