Strain Information

Name BFF68   View On Wormbase
Species C. elegans
GenotypemjIs134 II; hrde-1(pig6[degron::ha::hrde-1]) III.
DescriptionmjIs134 [mex-5p::GFP::(Gly)5Ala::his-58::tbb-2 3'UTR + Cbr-unc-119(+)] II. N-terminal Auxin-Inducible Degron and HA tag inserted into the endogenous hrde-1 locus. gfp fluorescence in germline (mex-5::gfp). Control strain for BFF69; does not carry TIR1 transgene necessary for the degradation. hrde-1(pig6) crRNA sequence: CAUAAUUUUGUCGAGCAAGU. Reference: Toker IA, et al. Dev Cell. 2022 Feb 7;57(3):298-309.e9. PMID: 35134343.
Made byOlga Antonova
Laboratory BFF
Reference Toker, I. A., Lev, I., Mor, Y., Gurevich, Y., Fisher, D., Houri-Zeevi, L., ... & Rechavi, O. (2020). Transgenerational Regulation of Sexual Attractiveness in C. elegans Nematodes. bioRxiv.
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