Strain Information
| Name | BCN9071 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | vit-2(crg9070[vit-2::gfp]) X. |
| Description | GFP knocked into C terminal of vit-2 gene by CRISPR/Cas9 using self-excising drug selection cassette method of Dickinson et al. (2015, Genetics). Strain is derived from BCN9070, which was outcrossed to remove a linked background mutation on LG X causing transgenic males to mate poorly. Reference: Perez MF, et al. Nature. 2017 Dec 7;552(7683):106-109. |
| Mutagen | CRISPR/Cas9 homologous recombination |
| Outcrossed | x8 |
| Made by | Marcos Francisco Perez |
| Laboratory | BCN |
| Reference | Perez, M. F., Francesconi, M., Hidalgo-Carcedo, C., & Lehner, B. (2017). Maternal age generates phenotypic variation in Caenorhabditis elegans. Nature, 552(7683), 106. |
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