Laboratory Information

NameYA View on WormBase
Allele designationyp
HeadShawn Ahmed
InstitutionUniversity of North Carolina, Chapel Hill, NC
Address Coker Hall
University of North Carolina
Department of Genetics
Chapel Hill 27599-3280
United States
Website http://www.bio.unc.edu/ahmed/lab/
Gene classes est  pot 

Strains contributed by this laboratory

Strain Genotype Species Description
temp_name308 hpr-1 Not stable - Mortal germline
YA1039 mrt-1(yp2) I. C. elegans yp2 mutation perturbs the MRT-1 DNA-binding domain. Mortal germline. Progressive telomere shortening due to telomerase dysfunction. Hypersensitive to DNA interstrand crosslinking agents. This strain will become sterile after propagating 10-20 generations. mrt-1 mutants can be rejuvenated by outcrossing. Reference: Meier B, et al. EMBO J. 2009 Nov 18;28(22):3549-63.
YA1059 trt-1(ok410) I. C. elegans Becomes very sick if starved for extended periods of time or if frozen and thawed repeatedly. Primer sequences provided by the C. elegans Reverse Genetics Core Facility for ok410 are: Outer Left Sequence: AAGACTGTCAGCTGGTGGGT. Outer Right Sequence: TTAGCCTTACATTGCCGCTT. Inner Left Sequence: TCATGCGCAACATCTAAAGC. Inner Right Sequence: CGAATAAAGCAATTTCCCGA. Inner primer WT PCR product: 2825.
YA1080 hpr-17(tm1579) II. C. elegans Defective in responding to ionizing radiation or hydroxyurea. Defective for telomerase activity in vivo. Freeze immediately and thaw when needed; becomes inviable if maintained as a homozygote. Received new stock 11/2008.
YA1116 mrt-1(tm1354) I. C. elegans Null allele. Mortal germline. Progressive telomere shortening due to telomerase dysfunction. Hypersensitive to DNA interstrand crosslinking agents. This strain will become sterile after propagating 10-20 generations. mrt-1 mutants can be rejuvenated by outcrossing. Reference: Meier B, et al. EMBO J. 2009 Nov 18;28(22):3549-63.
YA1197 ypIn2 II. C. elegans ypIn2 [daz-1p::pot-1::mCherry::tbb-2 3'UTR + Cbr-unc-119(+)] II. Single copy MosSCI insertion at ttTi5605 locus on LG II. Reference: Shtessel L, et al. G3 (Bethesda). 2013 Feb;3(2):305-13.
YA893 mrt-1(e2662) I. C. elegans Null allele. Mortal germline. Progressive telomere shortening due to telomerase dysfunction. Hypersensitive to DNA interstrand crosslinking agents. This strain will become sterile after propagating 10-20 generations. mrt-1 mutants can be rejuvenated by outcrossing. Reference: Meier B, et al. EMBO J. 2009 Nov 18;28(22):3549-63. [NOTE: allele is incorrectly described as e2661 in this publication.]

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
yp2 Allele substitution