Laboratory Information
| Name | WIN View on WormBase |
|---|---|
| Allele designation | jog |
| Head | Peter Duchek |
| Institution | Institute of Molecular Biotechnology of the Austrian Academy of Sciences |
| Address | IMBA Dr-Bohr-Gasse 3 Vienna 1030 Austria |
| Website | https://www.oeaw.ac.at/imba/scientific-facilities/fly-worm-facility |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| WIN179 | wago-4(jog179[PH::3xFlag::GFP::wago-4 *gg620]) II. | C. elegans | Temperature sensitive: maintenance at 15-20C. Reduced number of progeny at elevated temperatures. PH domain added to the 3xFLAG::GFP-tagged endogenous wago-4 locus in parental strain YY1325. This allele localizes WAGO-4 to the plasma membrane, but de-localization is not complete (some residual germ granule signal is detected). |
| WIN180 | wago-4(jog180[mts::3xFlag::GFP::wago-4 *gg620]) II. | C. elegans | Temperature sensitive: maintain at 15-20C. Reduced number of progeny and mortal germline phenotype at elevated temperatures. Mitochondria Targeting Sequence (mts) added to the 3xFLAG::GFP-tagged endogenous wago-4 locus localizes tagged WAGO-4 to mitochondria. This allele was introduced into 3xFlag::GFP tagged endogenous wago-4 locus in parental strain YY1325. |
| WIN253 | glh-4(jog16) glh-2(jog49) glh-1(jog50) I. | C. elegans | Temperature sensitive: maintain at 15-20C. Reduced number of progeny and mortal germline phenotype at elevated temperatures. Engineered mutations of FG repeats in three Vasa like GLH proteins (GLH-1, GLH-2 and GLH-4). glh-1(jog50) is F to A substitution. glh-4(jog16) is F to A substitution. glh-2(jog49) is a deletion of the FG repeats. This strain exhibits a subtle change in germ granule composition while maintaining their architecture. WAGO-4 is mislocalized to the cytoplasm leading to compromised function. |
| WIN272 | wago-4(jog272[3xFLAG::GFP::wago-4(Y611E) *gg620]) II. | C. elegans | Temperature sensitive: maintain at 15-20C. Reduced number of progeny at elevated temperatures. wago-4(jog272) is an engineered Y611E point mutation predicted to disrupt small RNA binding and exhibits a diffuse cytoplasmic localization. This allele was introduced into 3xFlag::GFP tagged endogenous wago-4 locus in parental strain YY1325. |
This laboratory hasn't submitted any alleles to the CGC.