Laboratory Information
| Name | PFR View on WormBase |
|---|---|
| Allele designation | |
| Head | Francesca Palladino |
| Institution | Ecole Normale Superieure de Lyon, Lyon, France |
| Address | LBMC ENS CNRS UMR 5239 9 Rue du Vercors Lyon 69007 France |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| ANA65 | adeIs1 II; unc-119(ed3) III. | C. elegans | adeIs1 [mex-5::spd-1::GFP + unc-119(+)] II. The transgene has been inserted on chromosome II, using the MosSCI technique. This strains expresses the SPD-1 protein fused to GFP in the germline (both males and females) and in embryos. SPD-1 is nucleolar in interphase and labels the central spindle in mitosis and meiosis, later accumulating at the midbody. Reference: Nahaboo W, et al. Mol Biol Cell. 2015 Jun 1;26(11):2020-9. doi: 10.1091/mbc.E14-12-1577. |
| ANA72 | adeIs1 II; unc-119(ed3) III; ltIs37 IV. | C. elegans | adeIs1 [mex-5::spd-1::GFP + unc-119(+)] II. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Superficially wild-type. SPD-1::GFP and mCherry-tagged histones allow visualisation of chromatin with central spindle/midbody during cell divisions. Reference: Nahaboo W, et al. Mol Biol Cell. 2015 Jun 1;26(11):2020-9. |
| JU2902 | Mesorhabditis bifurcata wild isolate. | Mesorhabditis bifurcata | Mesorhabditis bifurcata wild isolate. Male-female strain: maintain by crossing. Typically very few males on plates (only 10%); might be necessary to transfer large chunks to the new plates to ensure enough males are transferred. Species exhibits unusual reproductive strategy found in Mesorhabditis worms, which includes atypical meiosis, atypical spermatogenesis, atypical sex ratio, and XY chromosomes in males. Mesorhabditis bifurcata are soil nematodes, easy to breed in the lab and can be frozen (Grosmaire & al. Science 2019, doi: 10.1126/science.aau0099.). |
| JU3143 | Mesorhabditis okuensis wild isolate. | Mesorhabditis okuensis | Mesorhabditis okuensis wild isolate. Male-female strain: maintain by crossing. Typically very few males on plates (only 10%); might be necessary to transfer large chunks to the new plates to ensure enough males are transferred. This species displays a striking reproductive system (described in Grosmaire & al. Science 2019. doi: 10.1126/science.aau0099). |
| PFR39 | hpl-2(tm1489) unc-49(e407) III. | C. elegans | Unc. Thermosensitive. Sterile at 25C. |
| PFR40 | hpl-2(tm1489) III. | C. elegans | Thermosensitive. Sterile at 25C. |
| PFR60 | hpl-1(tm1624) X. | C. elegans | Wild type phenotype. |
This laboratory hasn't submitted any alleles to the CGC.