Laboratory Information

NamePFR View on WormBase
Allele designation
HeadFrancesca Palladino
InstitutionEcole Normale Superieure de Lyon, Lyon, France
Address LBMC
9 Rue du Vercors
Lyon 69007
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
ANA65 adeIs1 II; unc-119(ed3) III. C. elegans adeIs1 [mex-5::spd-1::GFP + unc-119(+)] II. The transgene has been inserted on chromosome II, using the MosSCI technique. This strains expresses the SPD-1 protein fused to GFP in the germline (both males and females) and in embryos. SPD-1 is nucleolar in interphase and labels the central spindle in mitosis and meiosis, later accumulating at the midbody. Reference: Nahaboo W, et al. Mol Biol Cell. 2015 Jun 1;26(11):2020-9. doi: 10.1091/mbc.E14-12-1577.
ANA72 adeIs1 II; unc-119(ed3) III; ltIs37 IV. C. elegans adeIs1 [mex-5::spd-1::GFP + unc-119(+)] II. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Superficially wild-type. SPD-1::GFP and mCherry-tagged histones allow visualisation of chromatin with central spindle/midbody during cell divisions. Reference: Nahaboo W, et al. Mol Biol Cell. 2015 Jun 1;26(11):2020-9.
JU3143 Mesorhabditis okuensis wild isolate. Mesorhabditis okuensis Mesorhabditis okuensis wild isolate. Male-female strain: maintain by crossing. Typically very few males on plates (only 10%); might be necessary to transfer large chunks to the new plates to ensure enough males are transferred. This species displays a striking reproductive system (described in Grosmaire & al. Science 2019. doi: 10.1126/science.aau0099).
PFR39 hpl-2(tm1489) unc-49(e407) III. C. elegans Unc. Thermosensitive. Sterile at 25C.
PFR40 hpl-2(tm1489) III. C. elegans Thermosensitive. Sterile at 25C.
PFR60 hpl-1(tm1624) X. C. elegans Wild type phenotype.
This laboratory hasn't submitted any alleles to the CGC.