Laboratory Information

NamePFR View on WormBase
Allele designation
HeadFrancesca Palladino
InstitutionEcole Normale Superieure de Lyon, Lyon, France
Address LBMC
ENS CNRS UMR 5239
9 Rue du Vercors
Lyon 69007
France
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
ANA65 adeIs1 II; unc-119(ed3) III. C. elegans adeIs1 [mex-5::spd-1::GFP + unc-119(+)] II. The transgene has been inserted on chromosome II, using the MosSCI technique. This strains expresses the SPD-1 protein fused to GFP in the germline (both males and females) and in embryos. SPD-1 is nucleolar in interphase and labels the central spindle in mitosis and meiosis, later accumulating at the midbody. Reference: Nahaboo W, et al. Mol Biol Cell. 2015 Jun 1;26(11):2020-9. doi: 10.1091/mbc.E14-12-1577.
ANA72 adeIs1 II; unc-119(ed3) III; ltIs37 IV. C. elegans adeIs1 [mex-5::spd-1::GFP + unc-119(+)] II. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Superficially wild-type. SPD-1::GFP and mCherry-tagged histones allow visualisation of chromatin with central spindle/midbody during cell divisions. Reference: Nahaboo W, et al. Mol Biol Cell. 2015 Jun 1;26(11):2020-9.
JU2902 Mesorhabditis bifurcata wild isolate. Mesorhabditis bifurcata Mesorhabditis bifurcata wild isolate. Male-female strain: maintain by crossing. Typically very few males on plates (only 10%); might be necessary to transfer large chunks to the new plates to ensure enough males are transferred. Species exhibits unusual reproductive strategy found in Mesorhabditis worms, which includes atypical meiosis, atypical spermatogenesis, atypical sex ratio, and XY chromosomes in males. Mesorhabditis bifurcata are soil nematodes, easy to breed in the lab and can be frozen (Grosmaire & al. Science 2019, doi: 10.1126/science.aau0099.).
JU3143 Mesorhabditis okuensis wild isolate. Mesorhabditis okuensis Mesorhabditis okuensis wild isolate. Male-female strain: maintain by crossing. Typically very few males on plates (only 10%); might be necessary to transfer large chunks to the new plates to ensure enough males are transferred. This species displays a striking reproductive system (described in Grosmaire & al. Science 2019. doi: 10.1126/science.aau0099).
PFR39 hpl-2(tm1489) unc-49(e407) III. C. elegans Unc. Thermosensitive. Sterile at 25C.
PFR40 hpl-2(tm1489) III. C. elegans Thermosensitive. Sterile at 25C.
PFR60 hpl-1(tm1624) X. C. elegans Wild type phenotype.
This laboratory hasn't submitted any alleles to the CGC.