Laboratory Information
| Name | ML View on WormBase |
|---|---|
| Allele designation | mc |
| Head | Michel Labouesse |
| Institution | UMR7622, IBPS, Paris, France |
| Address | 7-9 quai Saint Bernard Bat C, 7e etage, case 24 7-9 quai Saint Bernard Paris 75005 France |
| Website | http://archive.igbmc.fr/recherche/Prog_DSC/Eq_MLabo/index_uk.html |
| Gene classes | ale eld lir rdy rga pros |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| AC257 | ppk-3(n2668) X. | C. elegans | Growth retardation, enlarged vacuoles (late endosomes and lysosomes) in intestine, epidermis, coelomocytes and pharynx. 27% embryonic lethality and 8% post-embryonic lethality. |
| ML1150 | mlc-4(or253)/qC1 [dpy-19(e1259) glp-1(q339)] III; mcEx401. | C. elegans | mcEx401 [mlc-4p::GFP::mlc-4(WT) + pie-1p::GFP::mlc-4(WT) + rol-6(su1006)]. Rollers. Apparent homozygous lethal deletion chromosome balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT Rol, and segregate WT Rol, sterile Dpy (qC1 homozygotes), late embryonic - early larval lethal (mlc-4 homozygotes) and Sterile Rollers. Pick WT Rol and check for correct segregation of progeny to maintain. Reference: Gally C et al. Development. 2009 Sep;136(18):3109-19. |
| ML1151 | mlc-4(or253)/qC1 [dpy-19(e1259) glp-1(q339)] III; mcEx402. | C. elegans | mcEx402 [mlc-4p::GFP::mlc-4(DD) + pie-1p::GFP::mlc-4(WT) + rol-6(su1006)]. Rollers. Apparent homozygous lethal deletion chromosome balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT Rol, and segregate WT Rol, sterile Dpy (qC1 homozygotes), late embryonic - early larval lethal (mlc-4 homozygotes) and Sterile Rollers. Pick WT Rol and check for correct segregation of progeny to maintain. Reference: Gally C et al. Development. 2009 Sep;136(18):3109-19. |
| ML1213 | rdy-4(mc42)/mIn1 [dpy-10(e128) mIs14] II. | C. elegans | Heterozygotes are WT and segregate WT, Dpys that are GFP+ in the pharynx, and dead L1 larvae that are translucent and often found away from the bacterial lawn (can be difficult to spot on the lawn). |
| ML1214 | pros-1(mc41)/dpy-17(e164) unc-32(e189) III. | C. elegans | Heterozygotes are WT and segregate WT, Dpy Uncs, and dead L2 larvae that are translucent and often found away from the bacterial lawn (can be difficult to spot on the lawn). [NOTE: mc41 was initially described as an allele in rdy-3, but the affected gene has since been identified as pros-1] |
| ML1651 | mcIs46. | C. elegans | mcIs46 [dlg-1::RFP + unc-119(+)]. Superficially Wild-type. Reference: Diogon M, et al., Development. 2007 Jul;134(13):2469-79. |
| ML1735 | mcIs50. | C. elegans | mcIs50 [lin-26p::vab-10(actin-binding domain)::GFP + myo-2p::GFP + pBluescript]. Reference: Gally C, et al., Development. 2009 Sep;136(18):3109-19. |
| ML2482 | noah-1(mc68[noah-1::mCherry]) I. | C.elegans | Superficially wild-type. Endogenous noah-1 locus tagged with mCherry. Reference: Vuong-Brender TTK, et al. Development. 2017 May 19. pii: dev.150383. |
| ML2501 | let-805(mc73[let-805::gfp + unc-119(+)]) unc-119(ed3) III. | C. elegans | Superficially wild-type. mc73 was generated by using Crispr/Cas9 to add a GFP tag to the endogenous let-805 locus and also insert a rescuing unc-119 transgene. Reference: Quentin S, et al. Development 2016 143: 160-173; doi: 10.1242/dev.126615. |
| ML2540 | nmy-1(mc82[nmy-1::gfp]) X. | C.elegans | Superficially wild-type. Endogenous nmy-1 locus tagged with GFP. Reference: Vuong-Brender TTK, et al. eLife 2017;6:e23866 DOI: 10.7554/eLife.23866. |
| ML2550 | git-1(mc86[git-1::histag::2xpreCissionCleavageSite::gfp) X. | C.elegans | Superficially wild-type. Reference: Vuong-Brender TTK, et al. Development 2017 : doi: 10.1242/dev.150383 |
| ML2615 | dlg-1(mc103[dlg-1::gfp]) X. | C.elegans | Superficially wild-type. Endogenous dlg-1 locus tagged with GFP. |
| ML2822 | unc-119(ed3) III; mcIs54 X. | C. elegans | mcIs54 [dpy-7p::spas-1::SL2(operon)::mCherry + unc-119(+)] X. Dpy. Expression of Spastin transgene depletes microtubules specifically in the hypodermis, creating a Dpy phenotype. Reference: Quintin S, et al. Development. 2016 Jan 1;143(1):160-73. |
| ML335 | dpy-2(e489) mcDf1 unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. | C. elegans | Heterozygotes are WT and segregate WT, dead eggs and DpyUncs. mcDf1 homozygotes arrest as dead eggs. |
| ML456 | ale-1(mc14)/spe-6(hc49) unc-25(e156) III. | C. elegans | Heterozygotes are WT and segregate WT, embryonic lethals (embryos fail to elongate) and Sterile Uncs. mc14 identified as a mutation that leads to abnormal lin-26 expression. |
| ML503 | tra-2(q276)/rol-6(e187) II; mcDf2/dpy-8(e130) X. | C. elegans | Segregates WT, males, Dpy, Rol, DpyRol, dead eggs (mcDf2). Use XX tra-2 males to move mcDf2 around. mcDf2 is an approx. 500 kb deficiency removing a large chunk between mec-2 and sup-7 (non-inclusive). |
| ML514 | che-14(ok193) I. | C. elegans | Animals are dye-filling negative. A bit sick with about 30% dying during larval development and the others displaying a number of defects in organs/tissues containing hypodermal cells (hypodermis, rectum, vulva, excretory system). |
| ML535 | mcIs17. | C. elegans | mcIs17 [lin-26::GFP +rol-6(su1006)]. Rollers. The GFP signal is average in embryos and rather weak in larvae. GFP is detected in the same cells as the endogenous LIN-26 protein, that is hypodermal cells (including rectal and vulval cells), support cells, and the omatic gonad precursors Z1/Z4. The signal is too weak to be detected in uterine cells. mcIs17 insertion site not mapped. |
| ML581 | lin-26(mc15) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. | C. elegans | Heterozygotes are WT and segregate dead eggs, DpyUncs and WT. lin-26(mc15) is a molecular null due to a deletion of most of lin-26 coding sequence. |
| ML610 | lir-1(mc33) mcDf3/mnC1 [dpy-10(e128) unc-52(e444)] II. | C. elegans | Heterozygotes are WT and segregate WT, DpyUnc and dead eggs. mcDf3 is a deficiency very closely linked to lir-1 - not separable - which removes approx. 250 kb. |
| ML653 | vab-10(mc44)/unc-75(e950) unc-101(m1) I. | C. elegans | Heterozygotes are WT and segregate WT, Uncs and dead eggs and larvae with severe body morphology defects that do not develop beyond the L2 stage. A few very rare mc44 larvae reach adulthood but they become sterile. mc 44 is a deletion affecting the downstream transcription unit of vab-10 named vab-10b. Fails to complement the vab-10 null reference allele vab-10(h1356), but complements the vab-10a alleles vab-10a(e698) and vab-10a(ju281). |
| ML691 | vab-10(ok817)/unc-75(e950) unc-101(m1) I. | C. elegans | Heterozygotes are WT and segregate WT, Uncs, and severely Lumpy arrested L1s (ok817). m1 can easily get lost through recombination. |
| ML732 | vha-5(mc38)/unc-24(e138) dpy-20(e1282) IV. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs, and dead L1 larvae that are translucent and often found away from the bacterial lawn (can be difficult to spot on the lawn). |
| ML743 | rdy-2(mc40)/sqt-3(sc63) him-5(e1467) unc-76(e911) V. | C. elegans | Heterozygotes are WT and segregate WT, Rol Uncs, and dead L2 larvae that are translucent and often found away from the bacterial lawn (can be difficult to spot on the lawn). |
| ML773 | rga-2(hd102)/hIn1 [unc-54(h1040)] I. | C. elegans | Heterozygotes are WT and segregate WT, dead eggs, and paralyzed Uncs. Stable. |
| ML846 | vha-5(mc38) IV; mcEx337. | C. elegans | mcEx337 [vha-5(+)::GFP + rol-6(su1006)]. Animals with the array are Rollers and GFP+ in the excretory canal. Animals which have lost the array are dead L1 larvae that are translucent and often found away from the bacterial lawn (can be difficult to spot on the lawn). |
| ML851 | vha-5(mc38) IV; mcEx342. | C. elegans | mcEx342[vha-5(E830Q)::GFP + rol-6(su1006)]. Animals with the array are slightly Dpy and are occasionally Rollers and GFP+ in the excretory canal. Animals which have lost the array are dead L1 larvae that are translucent and often found away from the bacterial lawn (can be difficult to spot on the lawn). |
| ML855 | +/szT1 [lon-2(e678)] I; ppk-3(mc46)/szT1 X. | C. elegans | Heterozygotes are WT and segregate WT, arrested szT1 aneuploids, Lon males, mc46 hemizygotes (WT males) and mc46 homozygotes. mc46 is a deletion that results in homozygotes which show enlarged vacuoles (late endosomes and lysosomes) and lay eggs with enlarged vacuoles that die at different stages of embyronic development. |
| ML916 | mcIs40. | C. elegans | mcIs40 [lin-26p::ABDvab-10::mCherry + myo-2p::GFP]. Reference: Gally C, et al. Development. 2009 Sep;136(18):3109-19. |
| ML93 | dpy-2(e489) lin-26(mc1) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. | C. elegans | Heterozygotes are WT and segregate WT, DpyUnc and dead eggs (mc1 homozygotes). mc1 is a strong loss of function mutation, but probably not null. mc1 is an embryonic lethal mutation with degeneration of hypodermal cells. |
| MT7531 | ppk-3(n2835) X. | C. elegans | n2835 animals show enlarged vacuoles in intestine, epidermis, coelomocytes and pharynx. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| mc14 | Allele | ||
| mc42 | Allele | ||
| mc41 | Allele | ||
| mc15 | Allele | insertion | |
| mc33 | Allele | deletion | |
| mc44 | Allele | deletion | |
| mc38 | Allele | deletion | |
| mc40 | Allele | deletion | |
| mc46 | Allele | deletion | |
| mc1 | Allele | substitution |