Laboratory Information

NameGA View on WormBase
Allele designationwu
HeadDavid Gems
InstitutionUniversity College, London, England
Address Institute of Healthy Ageing
Darwin Building, University College London
Gower Street
London WC1E 6BT
United Kingdom
Website http://www.ucl.ac.uk/~ucbtdag/
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
DR1565 daf-2(m596) III. C. elegans Class 2 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt).
DR1566 daf-2(m579) III. C. elegans Adults Age and Itt and temperature sensitive Unc (class 2 allele). m579 results in an amino acid substitution(R437C) in the extracellular portion of the DAF-2 receptor (specifically, the Cys-rich domain).
DR1567 daf-2(m577) III. C. elegans Class 1 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt).
DR1568 daf-2(e1371) III. C. elegans Class 1 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt).
DR1572 daf-2(e1368) III. C. elegans Maintain at 15C.  Class 1 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt).
DR1574 daf-2(e1391) III. C. elegans Class 2 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). Produces some dauers at 15C. CGC received new stock 10/25/00 from David Gems. Received new stock from Patrice Albert in the Riddle lab 6/04.
DR1942 daf-2(e979) III. C. elegans This strain forms 20% dauers at 15C. At 25C there occurs about 25% embryonic arrest and about 75% L1 arrest. The e979 mutation results in an amino acid substitution, C146Y, in the ligand-binding domain of the DAF-2 receptor. [CGC received new stock of DR1942 September 2002. Previous stock was probably m41 and not e979.] [June 2004: Patrice Albert has confirmed the mutation in this stock: Repeat of sequencing for CGC collection strain DR1942 [daf-2(e979)] is complete. The strain does carry a C146Y mutation (coding strand TGC to TAC) [Mutation position is at 143, not 146, based on the amino acid sequence shown in Wormbase for daf-2. It's the C in partial sequence EKRCGPI of Exon 5.].]
DR608 daf-2(m212) III. C. elegans Adults Age and Itt, but not Unc (severe class 1 allele). m212 results in an amino acid substitution(C883Y) in the extracellular portion of the DAF-2 receptor (specificaly, the FnIII2ID domain).
GA132 daf-16(mgDf50) I; daf-2(m646) III. C. elegans Maintain at 15C.
GA158 daf-16(mgDf50) I; daf-2(m65) III. C. elegans Maintain at 15C.
GA184 sod-2(gk257) I. C. elegans Slow growing. Reduced brood size. Hypersensitive to oxidative stress.
GA186 sod-3(tm760) X. C. elegans Superficially wild-type.
GA187 sod-1(tm776) II. C. elegans Superficially wild-type.
GA2001 wuIs305. C. elegans wuIs305 [myo-3p::Queen-2m]. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741.
GA2002 daf-2(e1370) III; wuIs305. C. elegans wuIs305 [myo-3p::Queen-2m]. Temperature sensitive dauer constitutive. Maintain at 15C. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741.
GA2003 daf-16(mgDf50) I; daf-2(e1370) III; wuIs305. C. elegans wuIs305 [myo-3p::Queen-2m]. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741.
GA416 sod-4(gk101) III. C. elegans Superficially wild-type.
GA468 geIs3 I. C. elegans geIs3 [sir-2.1(+) + rol-6(su1006)]. Rollers. This strain replaces LG100. Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5.
GA480 sod-2(gk257) I; sod-3(tm760) X. C. elegans Slow growing. Reduced brood size. Hypersensitive to oxidative stress.
GA503 sod-5(tm1146) II. C. elegans Superficially wild-type.
GA631 lin-15B&lin-15A(n765) X; wuIs177. C. elegans wuIs177 [ftn-1p::GFP + lin-15(+)]. GFP expression in the intestine. ftn-1p::GFP transgene shows moderate basal expression under standard culture conditions in an otherwise wild-type background, but is strongly induced by reduced insulin/IGF-1 signalling, reduced HIF signalling, and increased free iron levels. References: Ackerman D & Gems D. PLoS Genet. 2012;8(3):e1002498. Valentini S, et al. Mech Ageing Dev. 2012 May;133(5):282-90.
GA800 wuIs151. C. elegans wuIs151 [ctl-1(+) + ctl-2(+) + ctl-3(+) + myo-2p::GFP].  wuIs151 contains multiple copies of a PCR fragment containing the entire ctl-1 ctl-2 ctl-3 gene cluster.  Slow growing, especially at 25 degrees.  Raise at 20 degress or cooler.
GA801 wuIs152. C. elegans wuIs152 contains [sod-1(genomic) + rol-6(su1006)]. Rolls.
GA805 wuIs156. C. elegans wuIs156 contains [sod-2(genomic) + rol-6(su1006)]. Rollers.
GA905 unc-119(ed3) III; pkIs1642. C. elegans pkIs1642 [sir-2.1(+) + unc-119(+)]. Derived by outcrossing NL3908 to HT1593 (six times). Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5.
GA906 unc-119(ed3) III; pkIs1641. C. elegans pkIs1641 [unc-119(+)]. Derived by outcrossing NL3908 to HT1593 (six times). Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5.
GA916 dyf-x(wu250) I. C. elegans Dyf. Derived by crossing dye-filling mutant wu250 away from geIs3 (LG100 x N2 males). Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5.
JK1438 daf-2(m65)/qC1 [dpy-19(e1259) glp-1(q339)] III. C. elegans Heterozygotes are WT and segregate WT, non-conditional dauers, and Sterile Dpys. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JT193 daf-2(sa193) III. C. elegans Adults Age and Itt, but not Unc (class 1 allele). sa193 results in an amino acid substitution(A580T) in the extracellular portion of the DAF-2 receptor (specifically, the L2 domain).

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
wu250 Allele