Laboratory Information
| Name | GA View on WormBase |
|---|---|
| Allele designation | wu |
| Head | David Gems |
| Institution | University College, London, England |
| Address | Institute of Healthy Ageing Darwin Building, University College London Gower Street London WC1E 6BT United Kingdom |
| Website | http://www.ucl.ac.uk/~ucbtdag/ |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| DR1565 | daf-2(m596) III. | C. elegans | Class 2 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). |
| DR1566 | daf-2(m579) III. | C. elegans | Adults Age and Itt and temperature sensitive Unc (class 2 allele). m579 results in an amino acid substitution(R437C) in the extracellular portion of the DAF-2 receptor (specifically, the Cys-rich domain). |
| DR1567 | daf-2(m577) III. | C. elegans | Class 1 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). |
| DR1568 | daf-2(e1371) III. | C. elegans | Class 1 allele of daf-2. Temperature sensitive Daf-c. Maintain at 15C. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). |
| DR1572 | daf-2(e1368) III. | C. elegans | Maintain at 15C. Class 1 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). |
| DR1574 | daf-2(e1391) III. | C. elegans | Class 2 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). Produces some dauers at 15C. CGC received new stock 10/25/00 from David Gems. Received new stock from Patrice Albert in the Riddle lab 6/04. |
| DR1942 | daf-2(e979) III. | C. elegans | This strain forms 20% dauers at 15C. At 25C there occurs about 25% embryonic arrest and about 75% L1 arrest. The e979 mutation results in an amino acid substitution, C146Y, in the ligand-binding domain of the DAF-2 receptor. [CGC received new stock of DR1942 September 2002. Previous stock was probably m41 and not e979.] [June 2004: Patrice Albert has confirmed the mutation in this stock: Repeat of sequencing for CGC collection strain DR1942 [daf-2(e979)] is complete. The strain does carry a C146Y mutation (coding strand TGC to TAC) [Mutation position is at 143, not 146, based on the amino acid sequence shown in Wormbase for daf-2. It's the C in partial sequence EKRCGPI of Exon 5.].] |
| DR608 | daf-2(m212) III. | C. elegans | Temperature sensitive Daf-c. Maintain at 15C. Adults Age and Itt, but not Unc (severe class 1 allele). m212 results in an amino acid substitution(C883Y) in the extracellular portion of the DAF-2 receptor (specificaly, the FnIII2ID domain). |
| GA132 | daf-16(mgDf50) I; daf-2(m646) III. | C. elegans | Maintain at 15C. |
| GA158 | daf-16(mgDf50) I; daf-2(m65) III. | C. elegans | Maintain at 15C. |
| GA184 | sod-2(gk257) I. | C. elegans | Slow growing. Reduced brood size. Hypersensitive to oxidative stress. |
| GA186 | sod-3(tm760) X. | C. elegans | Superficially wild-type. |
| GA187 | sod-1(tm776) II. | C. elegans | Superficially wild-type. |
| GA2001 | wuIs305. | C. elegans | wuIs305 [myo-3p::Queen-2m]. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741. |
| GA2002 | daf-2(e1370) III; wuIs305. | C. elegans | wuIs305 [myo-3p::Queen-2m]. Temperature sensitive dauer constitutive. Maintain at 15C. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741. |
| GA2003 | daf-16(mgDf50) I; daf-2(e1370) III; wuIs305. | C. elegans | wuIs305 [myo-3p::Queen-2m]. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741. |
| GA416 | sod-4(gk101) III. | C. elegans | Superficially wild-type. |
| GA468 | geIs3 I. | C. elegans | geIs3 [sir-2.1(+) + rol-6(su1006)]. Rollers. This strain replaces LG100. Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5. |
| GA480 | sod-2(gk257) I; sod-3(tm760) X. | C. elegans | Slow growing. Reduced brood size. Hypersensitive to oxidative stress. |
| GA503 | sod-5(tm1146) II. | C. elegans | Superficially wild-type. |
| GA631 | lin-15B&lin-15A(n765) X; wuIs177. | C. elegans | wuIs177 [ftn-1p::GFP + lin-15(+)]. GFP expression in the intestine. ftn-1p::GFP transgene shows moderate basal expression under standard culture conditions in an otherwise wild-type background, but is strongly induced by reduced insulin/IGF-1 signalling, reduced HIF signalling, and increased free iron levels. References: Ackerman D & Gems D. PLoS Genet. 2012;8(3):e1002498. Valentini S, et al. Mech Ageing Dev. 2012 May;133(5):282-90. |
| GA800 | wuIs151. | C. elegans | wuIs151 [ctl-1(+) + ctl-2(+) + ctl-3(+) + myo-2p::GFP]. wuIs151 contains multiple copies of a PCR fragment containing the entire ctl-1 ctl-2 ctl-3 gene cluster. Slow growing, especially at 25 degrees. Raise at 20 degress or cooler. |
| GA801 | wuIs152. | C. elegans | wuIs152 contains [sod-1(genomic) + rol-6(su1006)]. Rolls. |
| GA805 | wuIs156. | C. elegans | wuIs156 contains [sod-2(genomic) + rol-6(su1006)]. Rollers. |
| GA905 | unc-119(ed3) III; pkIs1642. | C. elegans | pkIs1642 [sir-2.1(+) + unc-119(+)]. Derived by outcrossing NL3908 to HT1593 (six times). Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5. |
| GA906 | unc-119(ed3) III; pkIs1641. | C. elegans | pkIs1641 [unc-119(+)]. Derived by outcrossing NL3908 to HT1593 (six times). Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5. |
| GA916 | dyf-x(wu250) I. | C. elegans | Dyf. Derived by crossing dye-filling mutant wu250 away from geIs3 (LG100 x N2 males). Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5. |
| JK1438 | daf-2(m65)/qC1 [dpy-19(e1259) glp-1(q339)] III. | C. elegans | Heterozygotes are WT and segregate WT, non-conditional dauers, and Sterile Dpys. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JT193 | daf-2(sa193) III. | C. elegans | Adults Age and Itt, but not Unc (class 1 allele). sa193 results in an amino acid substitution(A580T) in the extracellular portion of the DAF-2 receptor (specifically, the L2 domain). |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| wu250 | Allele |