| nlp-73(sy1465) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of nlp-73.
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
left flanking sequence: GGTGCTCATCGCGACCACTGTGCTCATCGCCGAGT
right flanking sequence: CTCGTGTATTCTATAACCGATTCGACGGCGGGCTC
inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: GTTATAGAATACACGAGACT
Method Reference: G3 (Bethesda).
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