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Heterozygous. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ik7 homozygotes (Egl, Mel, but do not display adult-onset sterility). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Only heterozygous animals should propagate. aph-2(ik7) is a CRISPR-engineered full deletion of the aph-2 gene (3321 bp deletion). Reference: Brinkley DM, et al. Genetics. 2024 Jul 8;227(3):iyae076. doi: 10.1093/genetics/iyae076. PMID: 38717968. aph-2(ik7) is the first full deletion of the aph-2 gene (3321 bp deletion); it was generated by CRISPR/Cas9, as described in Brinkley et al. 2024 Homozygous aph-2(ik7) animals are Egl and Mel and do not display adult-onset sterility (see Brinkley et al, 2024). Only heterozygous animals from this strain will propagate.

njIs10 [glr-3::GFP]. RIA interneurons express soluble GFP.

njIs11[glr-3p::GFP + ges-1p::RFP]. RIA interneurons express soluble GFP.

njIs12[glr-3p::glr-1::GFP + glr-3p::RFP + ges-1p::RFP]. RIA interneurons express soluble GFP.

njIs9 [glr-3p::snb-1::venus + ofm-1::GFP] IV.

Reference: Miyara A, et al. PLoS Genet. 2011 May;7(5):e1001384.

Reference: Miyara A, et al. PLoS Genet. 2011 May;7(5):e1001384.

Reference: Kimata T, et al. Genetics. 2012 Jun;191(2):509-21.