| GG62 |
C. elegans |
emb-34(g62) III. Show Description
Temperature sensitive-maintain at 15C. Some growth at 20C. Does not grow at 25C.
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| CG625 |
C. elegans |
unc-103(n1213) pha-1(e2123) III; him-5(e1490) V; rgEx235. Show Description
rgEx235 [plc-3p::YFP + pha-1(+)]. Expresses YFP from a 4.4bk upstream plc-3 promoter. Maintain at 20C.
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| DG627 |
C. elegans |
emb-30(tn377) III. Show Description
Temperature sensitive. Maintain at 15C. Male mating stock.
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| EG5003 |
C. elegans |
unc-119(ed3) III; cxTi10882 IV. Show Description
Unc. Not caused by cxTi10882. EG5003 contains background mutations (partial deletion of pgp-6 and pgp-7 and a deletion close to cTel3x.1). EG6250 is an outcrossed version of this strain. Mos1 allele generated by NemaGENETAG consortium (Laurent Segalat).
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| EG6250 |
C. elegans |
unc-119(ed3) III; cxTi10882 IV. Show Description
Unc. Not caused by cxTi10882. Mos1 allele generated by NemaGENETAG consortium (Laurent Segalat). Grows best on HB101 bacteria. Reference: Frokjaer-Jensen C, et al., Nat Genet. 2008 Nov;40(11):1375-83.
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| GR2260 |
C. elegans |
cdo-1(mg622) X. Show Description
Can be maintained on OP50. Suppresses Moco-deficient larval lethality. Reference: Warnhoff K & Ruvkun G. Nat Chem Biol. 2019 Mar 25. doi: 10.1038/s41589-019-0249-y.
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| WIN179 |
C. elegans |
wago-4(jog179[PH::3xFlag::GFP::wago-4 *gg620]) II. Show Description
Temperature sensitive: maintenance at 15-20C. Reduced number of progeny at elevated temperatures. PH domain added to the 3xFLAG::GFP-tagged endogenous wago-4 locus in parental strain YY1325. This allele localizes WAGO-4 to the plasma membrane, but de-localization is not complete (some residual germ granule signal is detected).
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| WIN180 |
C. elegans |
wago-4(jog180[mts::3xFlag::GFP::wago-4 *gg620]) II. Show Description
Temperature sensitive: maintain at 15-20C. Reduced number of progeny and mortal germline phenotype at elevated temperatures. Mitochondria Targeting Sequence (mts) added to the 3xFLAG::GFP-tagged endogenous wago-4 locus localizes tagged WAGO-4 to mitochondria. This allele was introduced into 3xFlag::GFP tagged endogenous wago-4 locus in parental strain YY1325.
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| WIN272 |
C. elegans |
wago-4(jog272[3xFLAG::GFP::wago-4(Y611E) *gg620]) II. Show Description
Temperature sensitive: maintain at 15-20C. Reduced number of progeny at elevated temperatures. wago-4(jog272) is an engineered Y611E point mutation predicted to disrupt small RNA binding and exhibits a diffuse cytoplasmic localization. This allele was introduced into 3xFlag::GFP tagged endogenous wago-4 locus in parental strain YY1325.
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| YY1325 |
C. elegans |
wago-4(gg620[3xflag::gfp::wago-4]) II. Show Description
3xflag::gfp inserted into endogenous wago-4 locus using CRISPR/Cas9 engineering. 3xFLAG::GFP::WAGO-4 is partially functional in this strain. Reference: Wan G, et al. Nature. 2018 May;557(7707):679-683.
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| ZG629 |
C. elegans |
iaIs22. Show Description
iaIs22 [gcy-36p::GFP + unc-119(+)]. gcy-36::GFP is consistently expressed in AQR, PQR, URXL and URXR neurons.
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