| PS8789 |
C. elegans |
dmsr-1(sy1522) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of dmsr-1.
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
left flanking sequence: CCTATACATGCCTACTTATCAATATTTCTATGCGT
right flanking sequence: GTTGGGTACAATCGCTAATTTCTGTAACATCGTCG
inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TCAATATTTCTATGCGTGTT
Method Reference: G3 (Bethesda).
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| NQ915 |
C. elegans |
dmsr-1(qn45) V. Show Description
Pumps and moves 30 minutes after 30-minute 35 deg heat shock. Derived by outcrossing NQ792 to remove transgene. Reference: Iannacone M, et al. eLife 2017.
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| NQ792 |
C. elegans |
qnIs303 IV; dmsr-1(qn45) V. Show Description
qnIs303 [hsp-16.2p::flp-13 + hsp-16.2p::GFP + rab-3p::mCherry] IV. Pumps and moves 2 hours after heat induced flp-13 over-expression. Outcrossed 1x to NQ570. Reference: Iannacone M, et al. eLife 2017.
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| CHS1126 |
C. elegans |
dmsr-1(yum1734) V; dmsr-2(yum1735) I; dmsr-3(yum1736) II; dmsr-8(yum1737) V. Show Description
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.
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| PHX6333 |
C. elegans |
dmsr-1(syb6331 syb6333[FRT::dmsr-1 exons 2-3::FRT]) V. Show Description
Conditional knockout of dmsr-1 created via consecutive insertion of two FRT sites (GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC) flanking the second and third exons. The sequence within the two FRT sites is predicted to encode the first four transmembrane alpha helices. FLP recombination excises this sequence and introduces a frameshift, resulting in a likely molecular null allele of dmsr-1. Reference: Rossi L, et al. Curr Biol. 2025 Apr 20:S0960-9822(25)00355-0. doi: 10.1016/j.cub.2025.03.039. PMID: 40273913.
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| CHS1167 |
C. elegans |
dmsr-9(yum1992) dmsr-10(yum1993) dmsr-11(yum1994) dmsr-12(yum1995) dmsr-13(yum1996) dmsr-14(yum1997) dmsr-15(yum1998) dmsr-16(yum1999) V. Show Description
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.
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| PS8863 |
C. elegans |
dmsr-10(sy1548) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of dmsr-10;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: CCAGTGGCCAGATCCAGAAACTGGAGATGCTAAGG
Right flanking sequence: ACTTGGTTATTTCTCAATTTATTAATACAGTTGTAC
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : AACTGGAGATGCTAAGGACT
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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| PS8882 |
C. elegans |
dmsr-11(sy1551) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of dmsr-11;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: CTGAGGCTGCAATTAATAACGGAATTGTTTCCTTCA
Right flanking sequence: TGGACGCATTAGTAAAgtaatttaaactttagc
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : CTTTACTAATGCGTCCATGA
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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| PS8884 |
C. elegans |
dmsr-14(sy1553) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of dmsr-14;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: GATGTTTATTGCTGTTAGTGATTTCGGATGTGCAGT
Right flanking sequence: TACTGGTTTGATGCAATTATTTATAAGAAATTATTC
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : GATTTCGGATGTGCAGTTAC
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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| PS8890 |
C. elegans |
dmsr-12(sy1559) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of dmsr-12;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: CCGGCCGTTTCACTACTATATATTCACTTCCTTAG
Right flanking sequence: TTGTTTTGCATTTTTTGCAAATATTATGATTGTG
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : CAAAAAATGCAAAACAACTA
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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| PS8892 |
C. elegans |
dmsr-13(sy1561) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of dmsr-13;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: CACTGTGCCATATCCGGAGCCGGGCACCGATGAA
Right flanking sequence: GTTGGGAATAGCACGATTCCATGGTTGATTACTAC
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : AGCCGGGCACCGATGAAGTT
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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| PS8894 |
C. elegans |
dmsr-15(sy1563) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of dmsr-15;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: AAAAAACATGTCAGAAAATAAAAATCGCGGAGATA
Right flanking sequence: TTTCGGATTGTCAACAAAATTTGCTCGATTCAAATC
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : TAAAAATCGCGGAGATATTT
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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| PS8896 |
C. elegans |
dmsr-16(sy1565) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of dmsr-16;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: GTTTTAAGTTTCTTTGCAAATGCTCTTATCGCTATAG
Right flanking sequence: TTCTGGCAAACAAGGTTATGAGGATTTCTGGAAC
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : TGCTCTTATCGCTATAGTTC
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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