Search Strains

F56D1.6. Homozygous. Outer Left Sequence: AGCTCCTACCCCGTTCTGAC. Outer Right Sequence: ATGCTCAAGCACATCTGGTG. Inner Left Sequence: TCAGAATTTCTTGGGTTCATCA. Inner Right Sequence: TGGAAAGTGAAGGGTTTTCAG. Inner Primer PCR Length: 1173 bp. Deletion Size: 678 bp. Deletion left flank: CGCAAGGAGGAGCTATGATCATCACAAAAG. Deletion right flank: TATACCGATGGAATGAGTACATATGGATTT. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

hpIs190 [nmr-1p::D3cpv + lin-15(+)]. Strong fluorescent marker for Ca2+ imaging (FRET) AVA, AVE, AVD, RIM and a few other neurons. Construct includes 5.1 kb nmr-1 genomic promoter sequence upstream of the ATG start codon but a excludes a 2 kb fragment encoding cex-1 that interferes with calcium imaging (Kawano and Zhen, unpublished observation). Reference: Kawano T, et al. Neuron. 2011 Nov 17;72(4):572-86. PMID: 22099460

hpIs327 [cex-1p::tomm-20::miniSOG::sl2::Cherry]. Maintain in darkness. miniSOG and Cherry expression in RIM.