Search Strains

K02F3.4. External left primer: ATTGTTCAGCTATCGGCTCC. External right primer: AGCTGCTGGAGTTGGAGGTA. Internal left primer: ACTCACCGCTCCAGGATG. Internal right primer: TGAGGTTGGTGAATAAGGGA. Internal WT amplicon: 1145 bp. Deletion size: 476 bp. Deletion left flank: GACACGACGTTCCCTACCGAAAGCGGACCG. Deletion right flank: AATTTAAACATTAAAAACCATTTCAGTTCA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

jyEx4 [zip-2::GFP + myo-2p::mCherry]. Pick animals with mCherry+ pharynx to maintain. Array is stable; might have integrated. ZIP-2::GFP protein is only expressed in the intestine when animals are infected with pathogenic Pseudomonas aeruginosa strain (PA14) or when other processes (e.g. translation) are perturbed (see Dunbar TL, et al. for more information). Reference: Dunbar TL, et al. Cell Host Microbe. 2012 Apr 19;11(4):375-86.

jyEx6 [zip-2::GFP + myo-2p::mCherry]. Pick animals with mCherry+ pharynx to maintain. zip-2p::GFP reporter is constitutively on. Reference: Dunbar TL, et al. Cell Host Microbe. 2012 Apr 19;11(4):375-86.

wgIs432 [zip-2::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)