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RW6011 C. elegans unc-52(st546) II; mnDp34 (II;f). Show Description
Maintain by picking WT animals and checking that they throw PATs. st546 is a recessive lethal which causes a "severe" PAT phenotype. Most PATs hatch and are easy to spot as misshapen 2-fold larvae. Strain will break down, so be sure to check that the WT are throwing PATs.
SHG2672 C. elegans gld-2(ust546[gld-2::GFP::3xFlag]) I. Show Description
GFP::3xFlag inserted into endogenous gld-2 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.