Search Strains

F12E12.5. External left primer: CTTCATCTCGCACTGACCAA. External right primer: ACAGTTGGCAGCTGGTCTTC. Internal left primer: AGAGCTTGAGCTTCAGGCAG. Internal right primer: GACGCCGTATTGTCGCTTAT. Internal WT amplicon: 1748 bp. Deletion size: 642 bp. Deletion left flank: TCCTGAAGAGCTTTTGAGTTGATGAAAGTG. Deletion right flank: AAATTGCTGGAATTGAACAGGAAAAATGGA. Insertion Sequence: ACA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

wgIs670 [sdz-12::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org).