Strain Information

Name WBM1344   View On Wormbase
Species C. elegans
Genotyperpl-22(wbm58[3xFLAG::rpl-22]) II.
Description3xFlag tag inserted at N-temrinus of endogenous rpl-22 locus. Can be used for Single-copy Knock-In Translating Ribosome Immunoprecipitation (SKI TRIP) experiments.
MutagenCrispr/Cas9
Outcrossedx6
Made byAnne Lanjuin
Laboratory WBM
Reference Wester, L.E., Lanjuin, A., Bruckisch, E.H.W., Perez-Matos, M.C., Stine, P.G., Heintz, C., Denzel, M.S., and Mair, W.B. (2023) A single-copy knockin translating ribosome immunoprecipitation toolkit for tissue-specific profiling of actively translated mRNAs in C. elegans. Cell Rep. Methods 3, 100433. 10.1016/j.crmeth.2023.100433
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